The Journal of nutrition · 2026
Combination of Tart Cherry and Fish Oil Exerted Stronger Attenuation of High-Fat Diet-Induced Gut Dysbiosis and Dysfunction in Females than in Males.
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Seifishahpar Maryam, Kim Jung Han, Abdelrahman Mostafa, Kalupahana Nishan Sudheera, Rohde Ana, Kandasamy Aaranyah, Thevasagayampillai Shiyanth, Castillo Micah, Gunaratne Preethi H, Moustaid-Moussa Naima
Аннотация
BACKGROUND: Gut dysbiosis, disrupted intestinal barrier integrity, and elevated bacterial LPS levels are hallmarks of obesity-related metabolic dysfunctions. Fish oil (FO) and tart cherry (TC) have individually been shown to modulate gut microbiota dysbiosis and improve gut integrity.
OBJECTIVES: We hypothesized that TC and FO, individually or in combination, would mitigate high-fat (HF) diet-induced dysbiosis and favorably modulate the expression of genes associated with intestinal barrier integrity.
METHODS: Male and female C57BL/6J (BL6) and TALLYHO/Jng (TH) mice were fed low-fat, HF, or HF supplemented with TC, FO, or TC+FO diets for 14 wk from weaning. Cecal DNA was analyzed using 16S rRNA sequencing, and intestinal tissue was assessed for gut integrity gene expression.
RESULTS: Supplementation with TC, FO, or TC+FO modulated gut microbial β-diversity in a strain- and sex-dependent manner. Combined TC+FO supplementation significantly increased Lachnospiraceae abundance (P < 0.03) and reduced Deferribacteraceae (P < 0.03), especially in females. With regard to intestinal barrier integrity, TC and FO supplementation, particularly in combination, attenuated HF-induced reductions in barrier integrity markers. TC+FO significantly upregulated Zo1, Ocln, and Muc2 expression in BL6 females (P < 0.05), whereas FO and TC+FO increased expression of these genes in TH females (P < 0.05), suggesting additive benefits.
CONCLUSIONS: Supplementation with TC and FO, particularly in combination, attenuated HF diet-induced gut dysbiosis and favorably modulated the expression of intestinal barrier-associated genes, suggesting potential protective effects against HF diet-induced gut dysfunction.
OBJECTIVES: We hypothesized that TC and FO, individually or in combination, would mitigate high-fat (HF) diet-induced dysbiosis and favorably modulate the expression of genes associated with intestinal barrier integrity.
METHODS: Male and female C57BL/6J (BL6) and TALLYHO/Jng (TH) mice were fed low-fat, HF, or HF supplemented with TC, FO, or TC+FO diets for 14 wk from weaning. Cecal DNA was analyzed using 16S rRNA sequencing, and intestinal tissue was assessed for gut integrity gene expression.
RESULTS: Supplementation with TC, FO, or TC+FO modulated gut microbial β-diversity in a strain- and sex-dependent manner. Combined TC+FO supplementation significantly increased Lachnospiraceae abundance (P < 0.03) and reduced Deferribacteraceae (P < 0.03), especially in females. With regard to intestinal barrier integrity, TC and FO supplementation, particularly in combination, attenuated HF-induced reductions in barrier integrity markers. TC+FO significantly upregulated Zo1, Ocln, and Muc2 expression in BL6 females (P < 0.05), whereas FO and TC+FO increased expression of these genes in TH females (P < 0.05), suggesting additive benefits.
CONCLUSIONS: Supplementation with TC and FO, particularly in combination, attenuated HF diet-induced gut dysbiosis and favorably modulated the expression of intestinal barrier-associated genes, suggesting potential protective effects against HF diet-induced gut dysfunction.