Detection of thyrotrophin releasing hormone in rat brain in vivo using novel antibody microprobes: effects of amphetamine.

Antibody microprobes of novel design were used to monitor thyrotrophin releasing hormone (TRH) in rat brain before and after-parenteral administration of amphetamine. Specific antibodies to TRH were bound to the outside of glass microprobes by adsorption to a surface of activated charcoal embedded in epoxylite resin. In male Wistar rats anaesthetised with chloral hydrate a series of antibody microprobes were implanted in forebrain. Amphetamine (10 mg/kg i.p.) caused a highly significant decrease in the binding of 125I-TRH to microprobes, indicating an increase in extracellular TRH localised in the lateral septum. There was also evidence of TRH release in the septo-hypothalamic nucleus. Neither saline, nor amphetamine at 2 mg/kg were able to evoke changes in the release of TRH at any sites.