Natural product research · 2022
Metabolomic profiling and correlations of supercritical extracts of guarana.
СтатьяНазвание и аннотация: Перевод в очереди
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Marques Leila Larisa Medeiros, Ribeiro Fabianne Martins, Nakamura Celso Vataru, Simionato Ane Stefano, Andrade Galdino, Zielinski Acácio Antonio Ferreira, Carollo Carlos Alexandre, Silva Denise Brentan da, Oliveira Admilton Gonçalves de, Mello João Carlos Palazzo de
Аннотация
A previous optimization of supercritical extraction from guarana seeds was performed applying orthogonal array design (OA9(34)). The antioxidant and antimicrobial activities of these extracts, as well as metabolomic profiling and correlations from the compounds by statistical analysis were determined. Extracts 1 (40% ethanol; 20 min; 40 °C and 100 bar), 2 (40% methanol; 60 min; 40 °C and 200 bar), and 8 (40% methanol; 40 min; 60 °C and 100 bar) had the highest combined values of antioxidant capacity for the DPPH, FRAP, ABTS and xanthine oxidase system methods, and were identified by chemometric analysis. Similar chemical profiles of the extracts were obtained by LC-DAD-MS, and were identified: methyl-xanthine, (epi)catechin and dimers and trimers of type A and B proanthocyanidins. The heat map analysis showed positive correlation between antioxidant methods DPPH, FRAP and ABTS and with flavan-3-ols and proanthocyanidins. Extract 3 was active against Gram-negative and -positive bacteria and Candida tropicalis.