[Neuropeptide Y promotes the proliferation and differentiation of 3T3-L1 preadipocytes].

AIM: To observe the effect of neuropeptide Y on the proliferation and differentiation of 3T3L1 preadipocytes and detect the mechanics.

METHODS

3T3-L1 preadipocytes were induced to differentiation by the cocktail medium containing 3-isobutyl-1-methylxanthine (IBMX), dexamethasone and insulin. After 2 days, the cells were divided into 3 groups: the control group(without inducer), the routine group(insulin used as inducer) and the experiment group(10(-8), 10(-9), 10(-10) mol/L NPY used as inducer). At day 7 and 12, cell differentiation was observed by phase contrast microscope. At day 12, Oil red O staining was performed. Proliferation capacities of 3T3L1 cells were assessed by MTT. Western blot was used to know the protein level of peroxisome proliferator-activated receptor gamma (PPAR-γ) and The CCAAT/enhancer-binding protein-α(C/EBP-α).

RESULTS

NPY 10(-8) mol/L promotes the differentiation of 3T3L1 preadipocytes, 10(-9) mol/L, 10(-8) mol/L NPY increased 3T3-L1 cells proliferation. The protein levels of PPAR-γ and C/EBP-α were up regulated by 10(-8) mol/L NPY.

CONCLUSION

Neuropeptide Y promotes the differentiation and Proliferation of 3T3-L1 cells, this effect may be related to up-regulation of PPAR-γ and C/EBP-α.