Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2011
[Expression profiling of peroxisome proliferation activate receptor gamma2 and phosphorylation during adipogenic differentiation of adipose-derived stem cells].
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Sun Qin-ce, Xiao Jin-gang, Yang Xiao-juan, Tian Wei-dong
Аннотация
OBJECTIVE: To investigate PPARgamma2 expression and phosphorylation involved in adipogenic differentiations of Adipose-derived Stem Cells (ASCs) with an aim to reveal the possible mechanisms of adipose development.
METHODS: ASCs were obtained from the inguinal fat pads of GFP (green fluorescent protein) mice. The primary cells were cultured in histiocytic attachment cultivation. Adipogenic differentiation of the third generation of ASCs was induced with the conditional medium (alpha-MEM medium supplemented with 100 mL/L fetal bovine serum, 10(-6) mol/L dexamethasone, 10(-5) mol/L insulin, 0. 2 mmol/L indomethacin, and 0.5 mmol/L 3-isobutyl-1-methylxanthine). The levels of PPARgamma2 mRNA and the PPARgamma2 expression and phosphorylation at day 0, 1, 3, 5, 7, and 9 of induction were detected using RT-PCR, fluorescence-immunocytochemistry and western blot, respectively.
RESULTS: The expression of PPARgamma2 and phosphorylation were significantly up-regulated during adipogenic differentiation of ASCs.
CONCLUSION: PPARgamma2 is the key regulator of adipose development. The regulation of PPARgamma2 phosphorylation may promote adipogenic differentiation of ASCs.
METHODS: ASCs were obtained from the inguinal fat pads of GFP (green fluorescent protein) mice. The primary cells were cultured in histiocytic attachment cultivation. Adipogenic differentiation of the third generation of ASCs was induced with the conditional medium (alpha-MEM medium supplemented with 100 mL/L fetal bovine serum, 10(-6) mol/L dexamethasone, 10(-5) mol/L insulin, 0. 2 mmol/L indomethacin, and 0.5 mmol/L 3-isobutyl-1-methylxanthine). The levels of PPARgamma2 mRNA and the PPARgamma2 expression and phosphorylation at day 0, 1, 3, 5, 7, and 9 of induction were detected using RT-PCR, fluorescence-immunocytochemistry and western blot, respectively.
RESULTS: The expression of PPARgamma2 and phosphorylation were significantly up-regulated during adipogenic differentiation of ASCs.
CONCLUSION: PPARgamma2 is the key regulator of adipose development. The regulation of PPARgamma2 phosphorylation may promote adipogenic differentiation of ASCs.