Ophthalmic research · 2008
Mineralocorticoids restore quiescent morphology and reduce VEGF receptor expression in inflamed choroidal endothelial cells in vitro.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Fitzgerald Melinda, Evill Lauren, Banz Kelly, Carroll Simon, Rodger Jennifer
Аннотация
BACKGROUND/AIMS: While the glucocorticoid triamcinolone acetonide (9alpha-fluoro-16alpha-hydroxyprednisolone, TA) has been widely administered as a treatment of ocular inflammation, mineralocorticoids have not been tested for their efficacy.
METHODS: We assessed cellular morphology and actin distribution by immunomicroscopy and light microscopy, membrane permeability with transendothelial resistance and cell surface vascular endothelial growth factor receptor-1 (VEGF-R1) expression by flow cytometry.
RESULTS: Fludrocortisone acetate was more effective than TA in restoring quiescent morphology and reducing membrane permeability in phorbol-12-myristate-acetate (PMA)-stimulated choroidal endothelial cells (CECs). Each of the corticosteroids inhibited VEGF-R1 cell surface expression in PMA-responsive CECs.
CONCLUSION: Mineralocorticoids may be of potential use in reducing vascular permeability in ocular disease.
METHODS: We assessed cellular morphology and actin distribution by immunomicroscopy and light microscopy, membrane permeability with transendothelial resistance and cell surface vascular endothelial growth factor receptor-1 (VEGF-R1) expression by flow cytometry.
RESULTS: Fludrocortisone acetate was more effective than TA in restoring quiescent morphology and reducing membrane permeability in phorbol-12-myristate-acetate (PMA)-stimulated choroidal endothelial cells (CECs). Each of the corticosteroids inhibited VEGF-R1 cell surface expression in PMA-responsive CECs.
CONCLUSION: Mineralocorticoids may be of potential use in reducing vascular permeability in ocular disease.
Предметные рубрики MeSH
ActinsAnimalsCapillary PermeabilityCell Line, TumorCell SizeChoroidCortodoxoneDose-Response Relationship, DrugEndothelial CellsFlow CytometryGene Expression RegulationMacaca mulattaMineralocorticoidsRNA, MessengerReceptors, Vascular Endothelial Growth FactorTetradecanoylphorbol AcetateTriamcinolone Acetonide