Depression of h-reflex following carbonic anhydrase inhibition appears unrelated to changes in synaptic effectiveness.

Presynaptic inhibition (PI) of Ia afferents was examined as a possible contributor to the depression of the soleus H-reflex following carbonic anhydrase (CA) inhibition with Acetazolamide (ACZ). Ten males (aged 22-32) were studied in two randomized conditions, control and ACZ administration (250 mg 14, 8, and 2 h before testing) separated by at least one week. PI of soleus Ia afferents was indirectly assessed two ways: a conditioning stimulus of Ia afferents in the common peroneal nerve (N = 6), and heteronymous Ia facilitation from the quadriceps to soleus muscle (N = 4). Conditioning (C) of the soleus H-reflex (common peroneal nerve stimulation protocol) resulted in depression of the H-reflex in the supine and standing position compared to the test (T, unconditioned) H-reflex in the same position. This result was unaltered following ACZ treatment. C (heteronymous facilitation protocol) resulted in facilitation of the H-reflex in the supine, but not the standing position. This result was unaltered following ACZ treatment. It was concluded that the depression of the H-reflex following CA inhibition (present study; Brechue et al., 1997) appears to be unrelated to changes in the tonic level of PI of Ia afferents. The best hypothesis for the reduction in the H-reflex appears to be conduction block of the primary afferent fibers secondary to local increases in PCO2.