Journal of endodontics · 2014
Effects of deferoxamine on the repair ability of dental pulp cells in vitro.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Jiang Long, Peng Wei-Wei, Li Li-Fen, Du Rong, Wu Tian-Tian, Zhou Zhuo-Jun, Zhao Jun-Jun, Yang Ya, Qu Dong-Lin, Zhu Ya-Qin
Аннотация
INTRODUCTION: In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia. The purpose of this study was to investigate the effects of DFO on the repair ability of DPCs.
METHODS: DPCs were obtained by using a tissue explant technique in vitro and were treated with different concentrations of DFO or hypoxia culture for 2 days. The viability, proliferation, migration, and odontogenic differentiation of DPCs were assayed and analyzed. The expression of hypoxia-inducible factor 1-alpha (HIF-1α) was assessed through Western blotting.
RESULTS: Ten micromolars of DFO enhanced the expression of HIF-1α similarly to hypoxia and did not affect the viability of DPCs for 2 days. Furthermore, the proliferation, migration, and odontogenic differentiation of DPCs were promoted by DFO.
CONCLUSIONS: These results suggest that DFO might improve the repair ability of DPCs by HIF-1α.
METHODS: DPCs were obtained by using a tissue explant technique in vitro and were treated with different concentrations of DFO or hypoxia culture for 2 days. The viability, proliferation, migration, and odontogenic differentiation of DPCs were assayed and analyzed. The expression of hypoxia-inducible factor 1-alpha (HIF-1α) was assessed through Western blotting.
RESULTS: Ten micromolars of DFO enhanced the expression of HIF-1α similarly to hypoxia and did not affect the viability of DPCs for 2 days. Furthermore, the proliferation, migration, and odontogenic differentiation of DPCs were promoted by DFO.
CONCLUSIONS: These results suggest that DFO might improve the repair ability of DPCs by HIF-1α.
Предметные рубрики MeSH
AdolescentAlkaline PhosphataseCalcification, PhysiologicCell Culture TechniquesCell DifferentiationCell HypoxiaCell MovementCell ProliferationCell SurvivalCells, CulturedColoring AgentsDeferoxamineDental PulpFluorescent DyesHumansHypoxia-Inducible Factor 1, alpha SubunitOdontoblastsOsteocalcinRegenerationSiderophoresTetrazolium SaltsThiazolesYoung Adult