The Journal of pharmacy and pharmacology · 2012
Cistanche deserticola extract increases bone formation in osteoblasts.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Li Te-Mao, Huang Hsin-Chih, Su Chen-Ming, Ho Tin-Yun, Wu Chi-Ming, Chen Wen-Chi, Fong Yi-Chin, Tang Chih-Hsin
Аннотация
OBJECTIVES: We investigated the effect of Cistanche deserticola Ma. (CD) on bone formation by cultured osteoblasts.
METHODS: The mineralized nodule formation assay was used to examine the in-vitro effects of CD on bone formation. Alkaline phosphatase (ALP), bone morphogenetic proteins (BMP)-2 and osteopontin (OPN) mRNA expression was analysed by quantitative real-time polymerase chain reaction. The mechanism of action of CD extract was investigated using Western blotting. The in-vivo anti-osteoporotic effect of CD extract was assessed in ovariectomized mice.
KEY FINDINGS: CD extract had no effect on the proliferation, migration or wound healing of cultured osteoblasts, but increased ALP, BMP-2 and OPN mRNA and bone mineralization. Mitogen-activated protein kinase (MAPK) or nuclear factor (NF)-κB inhibitors reduced CD extract-induced bone formation and ALP, BMP-2 and OPN expression. However, CD extract did not affect osteoclastogenesis. In addition, CD extract prevented the bone loss induced by ovariectomy in vivo.
CONCLUSIONS: CD may be a novel bone formation agent for the treatment of osteoporosis.
METHODS: The mineralized nodule formation assay was used to examine the in-vitro effects of CD on bone formation. Alkaline phosphatase (ALP), bone morphogenetic proteins (BMP)-2 and osteopontin (OPN) mRNA expression was analysed by quantitative real-time polymerase chain reaction. The mechanism of action of CD extract was investigated using Western blotting. The in-vivo anti-osteoporotic effect of CD extract was assessed in ovariectomized mice.
KEY FINDINGS: CD extract had no effect on the proliferation, migration or wound healing of cultured osteoblasts, but increased ALP, BMP-2 and OPN mRNA and bone mineralization. Mitogen-activated protein kinase (MAPK) or nuclear factor (NF)-κB inhibitors reduced CD extract-induced bone formation and ALP, BMP-2 and OPN expression. However, CD extract did not affect osteoclastogenesis. In addition, CD extract prevented the bone loss induced by ovariectomy in vivo.
CONCLUSIONS: CD may be a novel bone formation agent for the treatment of osteoporosis.
Предметные рубрики MeSH
Alkaline PhosphataseAnimalsBlotting, WesternBone Morphogenetic Protein 2Bone ResorptionCalcification, PhysiologicCistancheFemaleMiceMice, Inbred ICRMitogen-Activated Protein KinasesNF-kappa BOsteoblastsOsteoclastsOsteogenesisOsteopontinOsteoporosisOvariectomyPhytotherapyPlant ExtractsProtein Kinase InhibitorsRNA, MessengerReal-Time Polymerase Chain Reaction