Shanghai kou qiang yi xue = Shanghai journal of stomatology · 2025
[The regulation of PERK on osteogenic differentiation of human periodontal ligament cells].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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Li Lifen, Du Rong, Jiang Long
Аннотация
PURPOSE: To explore whether RNA-activated protein kinase-like ER resident kinase(PERK) has a regulatory effect on the osteogenic differentiation of human periodontal ligament cells (PDLCs).
METHODS: PDLCs were primarily cultured, and siRNA was used to silence the expression of PERK in PDLCs. PDLCs were cultured in osteoblast differentiation-induction medium. Subsequently, the osteogenic differentiation capacity was evaluated using alkaline phosphatase(ALP) staining and alizarin red staining.
RESULTS: After osteogenic induction culture of PDLCs in vitro, ALP and alizarin red staining were enhanced, and the deposition of mineral salts increased. Meanwhile, the expression of phosphorylated PERK(p-PERK) in osteogenic PDLCs was higher than cells in the control group. Transfection of si-PERK significantly downregulated PERK protein expression in PDLCs (P＜0.05). ALP and alizarin red staining showed that the PERK-/- group had markedly lower ALP staining intensity and calcium salt deposition than the osteogenic induction group, along with decreased activating transcription factor 4(ATF4) protein expression.
CONCLUSIONS: PERK regulates the osteogenic differentiation of PDLCs.
METHODS: PDLCs were primarily cultured, and siRNA was used to silence the expression of PERK in PDLCs. PDLCs were cultured in osteoblast differentiation-induction medium. Subsequently, the osteogenic differentiation capacity was evaluated using alkaline phosphatase(ALP) staining and alizarin red staining.
RESULTS: After osteogenic induction culture of PDLCs in vitro, ALP and alizarin red staining were enhanced, and the deposition of mineral salts increased. Meanwhile, the expression of phosphorylated PERK(p-PERK) in osteogenic PDLCs was higher than cells in the control group. Transfection of si-PERK significantly downregulated PERK protein expression in PDLCs (P＜0.05). ALP and alizarin red staining showed that the PERK-/- group had markedly lower ALP staining intensity and calcium salt deposition than the osteogenic induction group, along with decreased activating transcription factor 4(ATF4) protein expression.
CONCLUSIONS: PERK regulates the osteogenic differentiation of PDLCs.