Shanghai kou qiang yi xue = Shanghai journal of stomatology · 2014
[Cytotoxicity of a novel endodontic treatment material iRoot BP Plus to human gingival fibroblasts].
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Shi Shuang, Bao Zhi-fan, Chen Xu, Zhang Dan-dan
Аннотация
PURPOSE: To compare the cytotoxicity of a novel endodontic treatment material iRoot BP Plus and mineral trioxide aggregate (MTA) to human gingival fibroblasts in vitro.
METHODS: Cultured human gingival fibroblasts were exposed to multiple concentrations of material elutes (no dilution, 1:2 dilution, and 1:5 dilution). The test material samples were immersed and incubated in the culture medium for 1, 3 or 7 days at 37 degrees centigrade. The proliferation rate was evaluated using methylthiazol tetrazolium (MTT) assay. Cell relative growth rates were presented as x±s. The data was statistically analyzed by factorial design ANOVA using SPSS 20.0 software package.
RESULTS: Cell relative growth rates of the eluates of iRoot BP Plus and MTA in different concentrations ranged from 77.31% to 113.82%. The cytotoxicity grade of both materials was 0 or 1 (no cytotoxicity). There was no significant difference in the relative growth rate in different concentrations of iRoot BP plus and MTA eluates under different elution times (F(concentration×time×material)=1.393, P=0.256).
CONCLUSIONS: Both iRoot BP Plus and MTA exhibit minimal level of cytotoxicity.
METHODS: Cultured human gingival fibroblasts were exposed to multiple concentrations of material elutes (no dilution, 1:2 dilution, and 1:5 dilution). The test material samples were immersed and incubated in the culture medium for 1, 3 or 7 days at 37 degrees centigrade. The proliferation rate was evaluated using methylthiazol tetrazolium (MTT) assay. Cell relative growth rates were presented as x±s. The data was statistically analyzed by factorial design ANOVA using SPSS 20.0 software package.
RESULTS: Cell relative growth rates of the eluates of iRoot BP Plus and MTA in different concentrations ranged from 77.31% to 113.82%. The cytotoxicity grade of both materials was 0 or 1 (no cytotoxicity). There was no significant difference in the relative growth rate in different concentrations of iRoot BP plus and MTA eluates under different elution times (F(concentration×time×material)=1.393, P=0.256).
CONCLUSIONS: Both iRoot BP Plus and MTA exhibit minimal level of cytotoxicity.