Journal of endodontics · 2012
Proliferation and multilineage potential of CXCR4-positive human dental pulp cells in vitro.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Jiang Long, Peng Wei-Wei, Li Li-Fen, Yang Ya, Zhu Ya-Qin
Аннотация
INTRODUCTION: Although the results of our previous studies showed that the stromal cell-derived factor (SDF)-1α-CXC chemokine receptor 4 (CXCR4) axis may play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4(+) DPCs) toward the damaged sites, the specific function of CXCR4(+) DPCs in the injured dental pulp was still unknown. The purpose of this study was to verify whether CXCR4(+) DPCs possessed stem cells properties so that we can understand their role in area of injury.
METHODS: CXCR4(+) DPCs were isolated from normal DPCs with magnetic-activated cell sorting. The characteristics of the cells from the 3 groups of cells (ie, CXCR4(+) DPCs, CXCR4(-) DPCs, or nonsorted DPCs) were analyzed in colony formation, proliferation, and multilineage differentiation including odontogenic and adipogenic lineages.
RESULTS: The results showed that CXCR4(+) DPCs were the most dominant population in colony formation, proliferation, alkaline phosphatase activity, calcium content, and adipogenic differentiation among the 3 groups.
CONCLUSIONS: CXCR4(+) DPCs may contain more stem cells than nonsorted DPCs.
METHODS: CXCR4(+) DPCs were isolated from normal DPCs with magnetic-activated cell sorting. The characteristics of the cells from the 3 groups of cells (ie, CXCR4(+) DPCs, CXCR4(-) DPCs, or nonsorted DPCs) were analyzed in colony formation, proliferation, and multilineage differentiation including odontogenic and adipogenic lineages.
RESULTS: The results showed that CXCR4(+) DPCs were the most dominant population in colony formation, proliferation, alkaline phosphatase activity, calcium content, and adipogenic differentiation among the 3 groups.
CONCLUSIONS: CXCR4(+) DPCs may contain more stem cells than nonsorted DPCs.
Предметные рубрики MeSH
AdipogenesisAlkaline PhosphataseAnthraquinonesAzo CompoundsCalcification, PhysiologicCalciumCell CountCell Culture TechniquesCell LineageCell ProliferationCells, CulturedChemokine CXCL12ChemotaxisCollagen Type IColoring AgentsCulture MediaDental PulpExtracellular Matrix ProteinsHumansMesenchymal Stem CellsOdontogenesisOsteocalcinPPAR gammaPhosphoproteinsReceptors, CXCR4SialoglycoproteinsStem CellsTetrazolium SaltsThiazolesDentin Sialophosphoprotein