Journal of endodontics · 2012
Effects of simvastain and enamel matrix derivative on Portland cement with bismuth oxide-induced growth and odontoblastic differentiation in human dental pulp cells.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Lee So-Youn, Min Kyung-San, Choi Gi-Woon, Park Jae-Hong, Park Sang-Hyuk, Lee Sang-Im, Kim Eun-Cheol
Аннотация
INTRODUCTION: We previously reported that bismuth oxide containing Portland cement (BPC) showed similar biocompatibility to Portland cement (PC) in periodontal ligament cells. However, the bioactivity of simvastatin and Emdogain (Biora AB, Malmö, Sweden) on BPC was not reported. The aim of this study was to evaluate the effects of simvastatin and Emdogain on BPC compared with mineral trioxide aggregate (MTA) in human dental pulp cells (HDPCs).
METHODS: Cell growth was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay. Differentiation was evaluated by alkaline phosphatase (ALP) activity, alizarin red staining, and reverse-transcriptase polymerase chain reaction.
RESULTS: The cell growth of HDPCs exposed to Emdogain and simvastatin plus BPC was superior to those administered BPC alone and similar to those that received MTA for 14 days. The simvastatin and Emdogain groups increased the odontogenic potential of the BPC group with respect to ALP activity, mineralization nodules, messenger RNA expression of ALP, osteopontin, osteocalcin, Runx2, and osterix.
CONCLUSIONS: These results suggest that simvastatin and Emdogain improved cell growth and the differentiation of the BPC group in HDPCs and may be useful ingredients in BPC as pulp-capping material.
METHODS: Cell growth was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay. Differentiation was evaluated by alkaline phosphatase (ALP) activity, alizarin red staining, and reverse-transcriptase polymerase chain reaction.
RESULTS: The cell growth of HDPCs exposed to Emdogain and simvastatin plus BPC was superior to those administered BPC alone and similar to those that received MTA for 14 days. The simvastatin and Emdogain groups increased the odontogenic potential of the BPC group with respect to ALP activity, mineralization nodules, messenger RNA expression of ALP, osteopontin, osteocalcin, Runx2, and osterix.
CONCLUSIONS: These results suggest that simvastatin and Emdogain improved cell growth and the differentiation of the BPC group in HDPCs and may be useful ingredients in BPC as pulp-capping material.
Предметные рубрики MeSH
Alkaline PhosphataseAluminum CompoundsAnthraquinonesBiocompatible MaterialsBismuthCalcification, PhysiologicCalcium CompoundsCell Culture TechniquesCell DifferentiationCell LineCell ProliferationCell SurvivalColoring AgentsCore Binding Factor Alpha 1 SubunitDental CementsDental Enamel ProteinsDental PulpDrug CombinationsHumansHydroxymethylglutaryl-CoA Reductase InhibitorsMaterials TestingOdontoblastsOdontogenesisOsteocalcinOsteopontinOxidesPulp Capping and Pulpectomy AgentsReverse Transcriptase Polymerase Chain ReactionSilicatesSimvastatinSp7 Transcription FactorTetrazolium SaltsThiazolesTranscription Factors