Journal of endodontics · 2009
Optimal cytocompatibility of a bioceramic nanoparticulate cement in primary human mesenchymal cells.
Сравнительное исследованиеСтатьяРандомизированное исследованиеНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
De-Deus Gustavo, Canabarro Antonio, Alves Gutemberg, Linhares Adriana, Senne Maria Isabel, Granjeiro Jose Mauro
Аннотация
INTRODUCTION: The aim of this study was to evaluate and compare the cytotoxic effects of BioAggregate (Innovative BioCaramix Inc, Vancouver, BC, Canada), a novel bioceramic nanoparticulate cement, on human mesenchymal cells. White Pro-Root MTA (Dentsply, Tulsa Dental, Tulsa, OK) was used as a reference for comparison.
METHODS: Fifty-six human maxillary incisor teeth were submitted to a step-back flaring technique and prepared for cytotoxicity assay in an in situ root-end filling experimental model. After retro filling, each root containing MTA, BioAggregate, or empty root canals (control) was exposed to culture media for 24, 48, or 72 hours, providing several extraction media. Mesenchymal cells were incubated with each extract medium for 24 hours, and toxicity was evaluated by three different parameters of cell survival and integrity on the same sample: XTT, neutral red, and crystal violet dye elution.
RESULTS: No statistically significant differences between MTA and BioAggregate were found in all the experimental periods (p > 0.05).
CONCLUSION: DiaRoot BioAggregate displayed in vitro compatibility similar to MTA.
METHODS: Fifty-six human maxillary incisor teeth were submitted to a step-back flaring technique and prepared for cytotoxicity assay in an in situ root-end filling experimental model. After retro filling, each root containing MTA, BioAggregate, or empty root canals (control) was exposed to culture media for 24, 48, or 72 hours, providing several extraction media. Mesenchymal cells were incubated with each extract medium for 24 hours, and toxicity was evaluated by three different parameters of cell survival and integrity on the same sample: XTT, neutral red, and crystal violet dye elution.
RESULTS: No statistically significant differences between MTA and BioAggregate were found in all the experimental periods (p > 0.05).
CONCLUSION: DiaRoot BioAggregate displayed in vitro compatibility similar to MTA.
Предметные рубрики MeSH
Aluminum CompoundsBiocompatible MaterialsCalcium CompoundsCell SurvivalCells, CulturedCeramicsColoring AgentsCulture MediaCulture Media, ConditionedDental CementsDrug CombinationsHumansMaterials TestingMesodermNanoparticlesOxidesRetrograde ObturationRoot Canal Filling MaterialsSilicatesTetrazolium SaltsThiazolesTime Factors