Nature communications · 2020
Clonal evolution of acute myeloid leukemia revealed by high-throughput single-cell genomics.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАПоддержка правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Morita Kiyomi, Wang Feng, Jahn Katharina, Hu Tianyuan, Tanaka Tomoyuki, Sasaki Yuya, Kuipers Jack, Loghavi Sanam, Wang Sa A, Yan Yuanqing, Furudate Ken, Matthews Jairo, Little Latasha, Gumbs Curtis, Zhang Jianhua, Song Xingzhi, Thompson Erika, Patel Keyur P, Bueso-Ramos Carlos E, DiNardo Courtney D, Ravandi Farhad, Jabbour Elias, Andreeff Michael, Cortes Jorge, Bhalla Kapil, Garcia-Manero Guillermo, Kantarjian Hagop, Konopleva Marina, Nakada Daisuke, Navin Nicholas, Beerenwinkel Niko, Futreal P Andrew, Takahashi Koichi
Аннотация
Clonal diversity is a consequence of cancer cell evolution driven by Darwinian selection. Precise characterization of clonal architecture is essential to understand the evolutionary history of tumor development and its association with treatment resistance. Here, using a single-cell DNA sequencing, we report the clonal architecture and mutational histories of 123 acute myeloid leukemia (AML) patients. The single-cell data reveals cell-level mutation co-occurrence and enables reconstruction of mutational histories characterized by linear and branching patterns of clonal evolution, with the latter including convergent evolution. Through xenotransplantion, we show leukemia initiating capabilities of individual subclones evolving in parallel. Also, by simultaneous single-cell DNA and cell surface protein analysis, we illustrate both genetic and phenotypic evolution in AML. Lastly, single-cell analysis of longitudinal samples reveals underlying evolutionary process of therapeutic resistance. Together, these data unravel clonal diversity and evolution patterns of AML, and highlight their clinical relevance in the era of precision medicine.