Photodiagnosis and photodynamic therapy · 2025
Temporal transition in urine and plasma porphyrin levels following 5-aminolevulinic acid administration in healthy adults.
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Iida Tomoki, Ota Urara, Takahashi Kiwamu, Ishizuka Masahiro, Higashikawa Fumiko
Аннотация
BACKGROUND: 5-aminolevulinic acid hydrochloride photodynamic screening (5-ALA-PDS) is a tumor screening system that utilizes blood and urinary porphyrins following the administration of 5-aminolevulinic acid (5-ALA) hydrochloride. Although 5-ALA-PDS has been extensively studied in various cancer species, healthy adults, it has not been systematically evaluated in healthy adults. This study examined differences in urinary and plasma 5-ALA and porphyrin concentrations in healthy adults, based on 5-ALA hydrochloride post-administration time, age and sex.
METHODS: We orally administered 5-ALA hydrochloride (100 or 300 mg/body) to 120 healthy adults. Using high-performance liquid chromatography (HPLC), we determined the concentrations of 5-ALA, uroporphyrin I (UPI), uroporphyrin III (UPIII), coproporphyrin I (CPI), coproporphyrin III (CPIII), and protoporphyrin IX (PpIX) in urine and plasma samples collected from the study participants before and at 4, 6, and 8 h after dosing. Urinary 5-ALA and porphyrins levels were adjusted for urinary creatinine levels.
RESULTS: The total amount of CPI and III (CPI + III) in the urine consistently exceeded that of other porphyrins throughout the study. The levels of 5-ALA and porphyrins peaked at 4 h after 5-ALA hydrochloride administration. Women exhibited significantly higher levels of 5-ALA, total UPI + III, and CPI + III than did men. In the plasma, 5-ALA and PpIX levels increased at 4 h post-administration.
CONCLUSIONS: This study demonstrated the importance of comparing porphyrin concentrations in urine and plasma between cancer patients and healthy adults, stratified by sex and age group, in order to develop 5-ALA-PDS as an accurate method for cancer diagnosis.
METHODS: We orally administered 5-ALA hydrochloride (100 or 300 mg/body) to 120 healthy adults. Using high-performance liquid chromatography (HPLC), we determined the concentrations of 5-ALA, uroporphyrin I (UPI), uroporphyrin III (UPIII), coproporphyrin I (CPI), coproporphyrin III (CPIII), and protoporphyrin IX (PpIX) in urine and plasma samples collected from the study participants before and at 4, 6, and 8 h after dosing. Urinary 5-ALA and porphyrins levels were adjusted for urinary creatinine levels.
RESULTS: The total amount of CPI and III (CPI + III) in the urine consistently exceeded that of other porphyrins throughout the study. The levels of 5-ALA and porphyrins peaked at 4 h after 5-ALA hydrochloride administration. Women exhibited significantly higher levels of 5-ALA, total UPI + III, and CPI + III than did men. In the plasma, 5-ALA and PpIX levels increased at 4 h post-administration.
CONCLUSIONS: This study demonstrated the importance of comparing porphyrin concentrations in urine and plasma between cancer patients and healthy adults, stratified by sex and age group, in order to develop 5-ALA-PDS as an accurate method for cancer diagnosis.