Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2010
[Immune response in BALB/c mice immunized with BCG expressing HBV truncated C gene and preS1 gene].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Yin Wen, Lü Xin, Lei Ying-feng, Yang Jing, Sun Meng-ning, Ma Yu, Lan Hai-yun, Wang Ai-qin
Аннотация
AIM: To study expressing of HBV truncated core and preS1 protein in BCG and to explore the effect of humoral and cellular immune response stimulated by the recombinant BCG.
METHODS: A shuttle vector was constructed and was transformed into BCG which including truncated Core gene and preS1 gene of HBV. The recombinant BCGs were analyzed with SDS-PAGE and Western blot. The three groups of BALB/c mice were immunized with saline, BCG, BCG-pDE22 and BCG-pDE22-CS1 respectively. Then the antibody titer and CTL effects were evaluated.
RESULTS: Compared with the control group, the recombinant BCG could express a new protein band of 24 kD which was consistent with the size of CS1 fused protein which displayed a good antigen-binding property by Western blot analysis. The antibody titer significantly increased and CTL effect apparently enhanced in the recombinant BCG immunized group.
CONCLUSION: BCG could be as live vector which carrying HBV related genes, which developing a novel vaccine against HBV.
METHODS: A shuttle vector was constructed and was transformed into BCG which including truncated Core gene and preS1 gene of HBV. The recombinant BCGs were analyzed with SDS-PAGE and Western blot. The three groups of BALB/c mice were immunized with saline, BCG, BCG-pDE22 and BCG-pDE22-CS1 respectively. Then the antibody titer and CTL effects were evaluated.
RESULTS: Compared with the control group, the recombinant BCG could express a new protein band of 24 kD which was consistent with the size of CS1 fused protein which displayed a good antigen-binding property by Western blot analysis. The antibody titer significantly increased and CTL effect apparently enhanced in the recombinant BCG immunized group.
CONCLUSION: BCG could be as live vector which carrying HBV related genes, which developing a novel vaccine against HBV.