Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2008
[Immunogenicity of DNA vaccine prime-BCG boost against Mycobacterium tuberculosis].
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Gao Lei, Bao Lang, Hao Mu, Zhang Hui-dong
Аннотация
AIM: To compare the immunogenicity of vaccine strategies about combined DNA prime-BCG boost and DNA or BCG vaccination only.
METHODS: BALB/c mice were divided into four groups each containing 6 mice, and all mice received three immunizations at 2 weeks interval. One group of mice was vaccinated with PBS (PBS group); one group was vaccinated twice at 0 week and 2 weeks with combined DNA expressing two mycobacterial antigens, ESAT-6 and antigen 85A respectively; and then boosted with BCG at 4 weeks (DNA/BCG group); another group was vaccinated twice with combined DNA at 0 week, 2 weeks and 4 weeks(DNA/DNA group); and the final group received PBS twice at 0 week and 2 weeks and then vaccinated with BCG at 4 weeks(BCG group). Specific IgG antibody in serum of mice was determined with indirect ELISA in 4 weeks, 6 weeks, 8 weeks respectively after final vaccination.The splenic lymphocytes of mice were separated and stimulated with PPD to measure their proliferation by XTT, and to evaluate the production of interferon-r(IFN-gamma) and IL-4 in cell suspensions of spleen cells by ELISA.
RESULTS: PPD could stimulate specific IgG responses in DNA/BCG, DNA/DNA and BCG groups (DNA/DNA group>DNA/BCG group and BCG group), and the most significant response occurred in 12 weeks; the splenic lymphocyte proliferation reactions and IFN-gamma and IL-4 production was detectable in DNA/BCG, DNA/DNA and BCG groups and DNA/BCG group induced significant higher production, especially in 12 weeks.
CONCLUSION: Priming with the combined DNA vaccines and boost with attenuated M. bovis vaccine (BCG) could enhance specific cell reactions and high level IFN-gamma compared with DNA or attenuated M. bovis vaccine alone.
METHODS: BALB/c mice were divided into four groups each containing 6 mice, and all mice received three immunizations at 2 weeks interval. One group of mice was vaccinated with PBS (PBS group); one group was vaccinated twice at 0 week and 2 weeks with combined DNA expressing two mycobacterial antigens, ESAT-6 and antigen 85A respectively; and then boosted with BCG at 4 weeks (DNA/BCG group); another group was vaccinated twice with combined DNA at 0 week, 2 weeks and 4 weeks(DNA/DNA group); and the final group received PBS twice at 0 week and 2 weeks and then vaccinated with BCG at 4 weeks(BCG group). Specific IgG antibody in serum of mice was determined with indirect ELISA in 4 weeks, 6 weeks, 8 weeks respectively after final vaccination.The splenic lymphocytes of mice were separated and stimulated with PPD to measure their proliferation by XTT, and to evaluate the production of interferon-r(IFN-gamma) and IL-4 in cell suspensions of spleen cells by ELISA.
RESULTS: PPD could stimulate specific IgG responses in DNA/BCG, DNA/DNA and BCG groups (DNA/DNA group>DNA/BCG group and BCG group), and the most significant response occurred in 12 weeks; the splenic lymphocyte proliferation reactions and IFN-gamma and IL-4 production was detectable in DNA/BCG, DNA/DNA and BCG groups and DNA/BCG group induced significant higher production, especially in 12 weeks.
CONCLUSION: Priming with the combined DNA vaccines and boost with attenuated M. bovis vaccine (BCG) could enhance specific cell reactions and high level IFN-gamma compared with DNA or attenuated M. bovis vaccine alone.