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DOI 10.1038/s41590-020-0782-6

Медицинская библиотека · Nature immunology · 2020

Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19.

Peng Yanchun, Mentzer Alexander J, Liu Guihai, Yao Xuan, Yin Zixi, Dong Danning, Dejnirattisai Wanwisa, Rostron Timothy, Supasa Piyada, Liu Chang, López-Camacho César, Slon-Campos Jose, Zhao Yuguang, Stuart David I, Paesen Guido C, Grimes Jonathan M, Antson Alfred A, Bayfield Oliver W, Hawkins Dorothy E D P, Ker De-Sheng, Wang Beibei, Turtle Lance, Subramaniam Krishanthi, Thomson Paul, Zhang Ping, Dold Christina, Ratcliff Jeremy, Simmonds Peter, de Silva Thushan, Sopp Paul, Wellington Dannielle, Rajapaksa Ushani, Chen Yi-Ling, Salio Mariolina, Napolitani Giorgio, Paes Wayne, Borrow Persephone, Kessler Benedikt M, Fry Jeremy W, Schwabe Nikolai F, Semple Malcolm G, Baillie J Kenneth, Moore Shona C, Openshaw Peter J M, Ansari M Azim, Dunachie Susanna, Barnes Eleanor, Frater John, Kerr Georgina, Goulder Philip, Lockett Teresa, Levin Robert, Zhang Yonghong, Jing Ronghua, Ho Ling-Pei, Oxford Immunology Network Covid-19 Response T cell Consortium, ISARIC4C Investigators, Cornall Richard J, Conlon Christopher P, Klenerman Paul, Screaton Gavin R, Mongkolsapaya Juthathip, McMichael Andrew, Knight Julian C, Ogg Graham, Dong Tao

СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАПеревод в очереди

Аннотация

The development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines and therapeutics will depend on understanding viral immunity. We studied T cell memory in 42 patients following recovery from COVID-19 (28 with mild disease and 14 with severe disease) and 16 unexposed donors, using interferon-γ-based assays with peptides spanning SARS-CoV-2 except ORF1. The breadth and magnitude of T cell responses were significantly higher in severe as compared with mild cases. Total and spike-specific T cell responses correlated with spike-specific antibody responses. We identified 41 peptides containing CD4+ and/or CD8+ epitopes, including six immunodominant regions. Six optimized CD8+ epitopes were defined, with peptide-MHC pentamer-positive cells displaying the central and effector memory phenotype. In mild cases, higher proportions of SARS-CoV-2-specific CD8+ T cells were observed. The identification of T cell responses associated with milder disease will support an understanding of protective immunity and highlights the potential of including non-spike proteins within future COVID-19 vaccine design.

Для цитирования: Peng Yanchun, Mentzer Alexander J, Liu Guihai, Yao Xuan, Yin Zixi, Dong Danning, Dejnirattisai Wanwisa, Rostron Timothy, Supasa Piyada, Liu Chang, López-Camacho César, Slon-Campos Jose, Zhao Yuguang, Stuart David I, Paesen Guido C, Grimes Jonathan M, Antson Alfred A, Bayfield Oliver W, Hawkins Dorothy E D P, Ker De-Sheng, Wang Beibei, Turtle Lance, Subramaniam Krishanthi, Thomson Paul, Zhang Ping, Dold Christina, Ratcliff Jeremy, Simmonds Peter, de Silva Thushan, Sopp Paul, Wellington Dannielle, Rajapaksa Ushani, Chen Yi-Ling, Salio Mariolina, Napolitani Giorgio, Paes Wayne, Borrow Persephone, Kessler Benedikt M, Fry Jeremy W, Schwabe Nikolai F, Semple Malcolm G, Baillie J Kenneth, Moore Shona C, Openshaw Peter J M, Ansari M Azim, Dunachie Susanna, Barnes Eleanor, Frater John, Kerr Georgina, Goulder Philip, Lockett Teresa, Levin Robert, Zhang Yonghong, Jing Ronghua, Ho Ling-Pei, Oxford Immunology Network Covid-19 Response T cell Consortium, ISARIC4C Investigators, Cornall Richard J, Conlon Christopher P, Klenerman Paul, Screaton Gavin R, Mongkolsapaya Juthathip, McMichael Andrew, Knight Julian C, Ogg Graham, Dong Tao. Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19.. Nature immunology. 2020. DOI: 10.1038/s41590-020-0782-6.

Предметные рубрики MeSH

Antigens, ViralBetacoronavirusCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCOVID-19COVID-19 VaccinesCoronavirus InfectionsEpitopes, T-LymphocyteHumansImmunodominant EpitopesImmunologic MemoryPandemicsPneumonia, ViralSARS-CoV-2Spike Glycoprotein, CoronavirusUnited KingdomViral Vaccines
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