Antiviral research · 2016
Recombinant DNA vaccine of Hantavirus Gn and LAMP1 induced long-term immune protection in mice.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Jiang Dong-Bo, Sun Li-Juan, Cheng Lin-Feng, Zhang Jin-Peng, Xiao Shao-Bo, Sun Yuan-Jie, Yang Shu-Ya, Wang Jing, Zhang Fang-Lin, Yang Kun
Аннотация
BACKGROUND: Prophylaxis is widely adopted the best choice against Hemorrhagic fever with renal syndrome (HFRS) caused by Hantavirus. However, loss of memory immune response maintenance remains as major shortcoming in current HFRS vaccine. A recombinant DNA vaccine, pVAX-LAMP/Gn was previously proved efficient, requiring long-term evaluations.
METHODS & RESULTS: Immune responses of Balb/c mice were assessed by specific and neutralizing antibodies, interferon-γ ELISpot assay, and cytotoxic T-lymphocyte cytotoxicity assay. HTNV-challenge assay identified long-term protection. Safety was confirmed by histological and behavioral analysis. Epitope-spreading phenomenon was noted, revealing two sets of dominant T-cell epitopes cross-species.
CONCLUSION: pVAX-LAMP/Gn established memory responses within a long-term protection. Lysosome-targeted strategy showed promise on Gn-based DNA vaccine and further investigations are warranted in other immunogenic Hantaviral antigens.
METHODS & RESULTS: Immune responses of Balb/c mice were assessed by specific and neutralizing antibodies, interferon-γ ELISpot assay, and cytotoxic T-lymphocyte cytotoxicity assay. HTNV-challenge assay identified long-term protection. Safety was confirmed by histological and behavioral analysis. Epitope-spreading phenomenon was noted, revealing two sets of dominant T-cell epitopes cross-species.
CONCLUSION: pVAX-LAMP/Gn established memory responses within a long-term protection. Lysosome-targeted strategy showed promise on Gn-based DNA vaccine and further investigations are warranted in other immunogenic Hantaviral antigens.
Предметные рубрики MeSH
AnimalsAntibodies, NeutralizingAntibodies, ViralDNADNA, RecombinantEnzyme-Linked Immunospot AssayOrthohantavirusHantavirus InfectionsImmunologic MemoryLysosomal Membrane ProteinsMembrane GlycoproteinsMiceMice, Inbred BALB CVaccines, DNAViral ProteinsViral VaccinesLysosomal-Associated Membrane Protein 1