[A method of production of concentrated and purified vaccine against Chlamydia abortion in sheep and its testing in guinea pigs].

A method was developed for the production of a new, present day, concentrated depot-vaccine against the chlamydial abortion of sheep. Employed were a number of methods -- those with chick embryos, freezing and thawing with the use of liquid nitrogen, inactivation with formalin, purifying and concentration through differential centrifugation and supercentrifugation, with the use of an aluminum hydroxide ingredient, electron microscopy, and the respective control series, etc. The vaccine is characterized by low dosing, complete innocuity, and unreactiveness. Guinea pigs immunized singly responded with the production of complement-fixing antibodies on the 30th day of an average titer ranging from 1:32 to 1:64. Two-fold immunization on the 1st and 30th day led to the production of antibodies of average titers within the 1:256-1:512 range as established by means of CFT. The vaccine was shown to have a low prime cost.