Hypoallergenic Derivatives of Tropomyosin from Oratosquilla oratoria Alleviate Allergy Responses via Regulating Th1/Th2 Balance.

Tropomyosin (TM), a major allergen in Oratosquilla oratoria with strong immunobinding activity, underscores the importance of allergen-specific immunotherapy, for which hypoallergenic derivatives represent a promising strategy. In this study, the derivatives of TM from O. oratoria were prepared by selective deletion of B-cell epitopes and retention of T-cell epitopes and were named mTM. The hypoallergenicity of mTM was further confirmed in a mouse model of food allergy. Furthermore, the prophylactic efficiency of mTM was evaluated in a prophylactic mouse model for O. oratoria allergy. It was shown that mTM simultaneously attenuated IgE-mediated allergic responses, alleviated allergic responses via regulating Th1/Th2 balance, and enhanced the ratio of regulatory T cell-associated cytokines. Overall, the study provides a theoretical basis and research direction for the development of hypoallergenic derivatives for prophylactic administration in food allergies.