Establishment of a passive-transfer mouse model of anti-NMDAR encephalitis and delineation of the PI3K-AKT-HIF-1α Axis in antibody-induced neuronal injury.

BACKGROUND AND

OBJECTIVE

Anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis is the most common autoimmune encephalitis. Currently, the development of its animal models lacks maturity and efficiency. This study aimed to establish a reliable and efficient mouse model of anti-NMDAR encephalitis and to investigate the pathological mechanisms.

METHODS

Immunoadsorption eluates were collected from three patients with anti-NMDAR encephalitis and three control patients. Immunoglobulin G (IgG) was purified from these eluates. A passive transfer mouse model was established by injecting the IgG into the hippocampal region of mice. RNA sequencing was performed on IgG treated neurons in vitro. And the role of the PI3K-AKT-HIF-1α signaling axis in NMDAR-IgG-induced neuronal injury was validated.

RESULTS

Compared with control-IgG, injection of NMDAR-IgG resulted in detectable antibody binding to hippocampal neurons in brain at 7 days post-injection, accompanied by loss of the postsynaptic scaffolding protein. Behavioral assessments revealed that NMDAR-IgG-treated mice exhibited cognitive impairment, depressive-like and despair-like behaviors on day 7, which had resolved by day 14. RNA sequencing of NMDAR-IgG-treated neurons in vitro identified dysregulation of the PI3K-AKT-HIF-1α signaling axis. Western blot demonstrated activation of this signaling axis in neurons following NMDAR-IgG treatment in both in vivo and in vitro. Additional experiments demonstrated that this signaling axis constitutes a critical pathway mediating NMDAR-IgG-induced neuronal injury. Finally, RNA sequencing and immunofluorescence confirmed microglial activation and increased HIF-1α secretion in the brains of NMDAR-IgG-treated mice.

CONCLUSION

We established a mouse model of anti-NMDAR encephalitis using antibodies purified from human immunoadsorption eluates. Furthermore, our findings demonstrate that the PI3K-AKT-HIF-1α signaling axis serves as a molecular pathway mediating NMDAR-IgG-induced neuronal injury.