Frontiers in immunology · 2026
Contrasted modifications of IgM and IgT repertoires induced by high- and low-virulent infectious pancreatic necrosis virus strains in rainbow trout (Oncorhynchus mykiss).
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Navelsaker Thommessen Sofie, Jouneau Luc, Castro Rosario, Mhanna Vanessa, Mariotti-Ferrandiz Encarnita, Munang Andu Hetron M, Gamil Amr Ahmed Abdelrahim, Collet Bertrand, Boudinot Pierre, Evensen Oystein
Аннотация
INTRODUCTION: Infectious pancreatic necrosis virus (IPNV) is a significant pathogen in salmonid aquaculture, with virulence determined mainly by amino acid residues in the VP2 capsid protein.
METHODS: This study investigates the modifications of rainbow trout (Oncorhynchus mykiss) IgM and IgT repertoire induced by two IPNV strains that differ at VP2 positions 217 and 221, specifically IPNV-TA (T217A221) and IPNV-PT (P217T221). For these studies, doubled-haploid isogenic trout were immunised with IPNV-TA or IPNV-PT, and IgHμ and IgHτ repertoires were analysed by high-throughput sequencing.
RESUTLS: We demonstrated that IPNV-PT elicited a strong and diverse modification if the IgM repertoire in the spleen, with significant clonotypic expansions including public components, particularly within VH1, VH4, and VH8 subgroups, observed two months post-immunization. Despite this robust response, clonotype usage largely reflected a non-convergent immune profile, consisting essentially of private responses, with limited sharing across individuals. In contrast, IPNV-TA triggered only modest changes in the repertoire. Notably, the IgM response to IPNV-PT diminished by four months, with limited persistence of public clonotypes. Changes of spleen IgT repertoire remained limited.
DISCUSSION: These findings demonstrate that even minor VP2 variations can profoundly affect not only the magnitude but also the clonal architecture and composition of antiviral B cell responses, highlighting the importance of detailed, strain-specific immune profiling in guiding vaccine development for aquaculture.
METHODS: This study investigates the modifications of rainbow trout (Oncorhynchus mykiss) IgM and IgT repertoire induced by two IPNV strains that differ at VP2 positions 217 and 221, specifically IPNV-TA (T217A221) and IPNV-PT (P217T221). For these studies, doubled-haploid isogenic trout were immunised with IPNV-TA or IPNV-PT, and IgHμ and IgHτ repertoires were analysed by high-throughput sequencing.
RESUTLS: We demonstrated that IPNV-PT elicited a strong and diverse modification if the IgM repertoire in the spleen, with significant clonotypic expansions including public components, particularly within VH1, VH4, and VH8 subgroups, observed two months post-immunization. Despite this robust response, clonotype usage largely reflected a non-convergent immune profile, consisting essentially of private responses, with limited sharing across individuals. In contrast, IPNV-TA triggered only modest changes in the repertoire. Notably, the IgM response to IPNV-PT diminished by four months, with limited persistence of public clonotypes. Changes of spleen IgT repertoire remained limited.
DISCUSSION: These findings demonstrate that even minor VP2 variations can profoundly affect not only the magnitude but also the clonal architecture and composition of antiviral B cell responses, highlighting the importance of detailed, strain-specific immune profiling in guiding vaccine development for aquaculture.