Analysis of the Effect of in vitro Hemolysis Test in the Treatment of Patients with Autoimmune Hemolytic Anemia.

OBJECTIVE

To investigate the efficacy of the in vitro hemolysis test in the treatment of patients with autoimmune hemolytic anemia (AIHA).

METHODS

This retrospective study analyzed data from AIHA patients treated at our hospital. Patients were categorized into two groups based on the blood-matching method recorded in their records: a control group and a research group. In the control group, the microcolumn gel method was applied for blood allocation and transfusion. In the research group, blood allocation and transfusion were guided by the in vitro hemolysis test.

RESULTS

After transfusion, hematological indices (RBC, TBil, Hb, Ret) improved in both groups compared to pre-transfusion values. The research group showed higher RBC and Hb levels, and lower TBil and Ret levels than the control group (P<0.05). Coagulation parameters (PT, APTT, FIB) also improved after transfusion. The research group had lower PT and APTT, and higher FIB than the control group (P<0.05). The total effective rate was higher in the research group (86.0% vs 66.7%, P<0.05).

CONCLUSIONS

The in vitro hemolysis assay for blood matching can improve hematological and coagulation parameters in AIHA patients and enhance transfusion efficacy, demonstrating significant clinical value.