Zhongguo shi yan xue ye xue za zhi · 2026
[Diversity of HPA Antigen-antibody and Its Application in Platelet Transfusion in Shenzhen Area].
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Xia Wen-Xia, Yang Fan, Che Hua-Tao, Li Xi-Miao, Gao Su-Qing, Xu Yun-Ping
Аннотация
OBJECTIVE: To investigate the polymorphism and genotype frequency of HPA gene in platelet donors in Shenzhen area, and to explore its application in clinical platelet transfusion.
METHODS: The blood samples of 461 platelet donors aged 18-55 years old were genotyped for HPA-1~6,-10,-15 and -21 using multiple fluorescent PCR. Platelet-specific antibodies were detected according to the instructions of PAKPLUS kit.
RESULTS: The a and b alleles of HPA-3 and HPA-15 genes showed high frequency distribution, and their gene frequencies were HPA-3a (54.23%), HPA-3b (45.77%), HPA-15a(55.42%),HPA-15b(44.58%), respectively, and their dual antigen mismatch rate was the highest (37.32%, 37.20%). In HPA-21, the genotype distribution between male and female was statistically significantly different( P < 0.05). Among the patients with positive PAKPLUS test, 35.59% of patients were positive for platelet membrane glycoprotein antibody.
CONCLUSION: Some HPA antigens in Shenzhen area have high mismatch rates and significant regional differences. The investigation of HPA genes and antibodies diversity laid the foundation for establishing a local platelet donor and recipient HPA information database and addressing platelet alloimmunity issues.
METHODS: The blood samples of 461 platelet donors aged 18-55 years old were genotyped for HPA-1~6,-10,-15 and -21 using multiple fluorescent PCR. Platelet-specific antibodies were detected according to the instructions of PAKPLUS kit.
RESULTS: The a and b alleles of HPA-3 and HPA-15 genes showed high frequency distribution, and their gene frequencies were HPA-3a (54.23%), HPA-3b (45.77%), HPA-15a(55.42%),HPA-15b(44.58%), respectively, and their dual antigen mismatch rate was the highest (37.32%, 37.20%). In HPA-21, the genotype distribution between male and female was statistically significantly different( P < 0.05). Among the patients with positive PAKPLUS test, 35.59% of patients were positive for platelet membrane glycoprotein antibody.
CONCLUSION: Some HPA antigens in Shenzhen area have high mismatch rates and significant regional differences. The investigation of HPA genes and antibodies diversity laid the foundation for establishing a local platelet donor and recipient HPA information database and addressing platelet alloimmunity issues.