Perspective on the application of genome sequencing for monkeypox virus surveillance.

• Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity. • The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained. • Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated. • Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.