Development and testing of a sampling device for the detection of aerosolized pathogens in indoor environments.

Effective airborne pathogen surveillance is essential for controlling the spread of respiratory infections in public spaces. A simple and effective sampling device, together with the validated sampling scheme and subsequent downstream analysis is needed. We developed and tested wet and dry air sampling concepts to efficiently capture aerosolized model microorganisms. Using Bacillus subtilis and feline infectious peritonitis virus (FIPV, a model for SARS-CoV-2), we evaluated capture efficiency via qPCR-based detection. The dry sampling concept based on the utilization of the intrinsic charge of aerosolized particles demonstrated superior efficiency. After determination of the correct electric field intensity and sampling time, the devices proved their routine applicability by successful sampling in lecture halls and a supermarket, where a higher fluctuation of people was expected. These findings have led to the development of a compact, mobile, and practical air sampling system for real-time pathogen monitoring, offering a valuable tool for epidemiological surveillance and infection control in high-occupancy environments.