Memorias do Instituto Oswaldo Cruz · 2025
IgY antibodies/Cysteamine: simple and effective methodology for electrochemical detection of SARS-CoV-2 S-protein.
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Gandarilla Ariamna, Romaguera-Barcelay Yonny, Glória Juliane Corrêa, Freire Luciana, Farias Taisa, Feitosa Jessica, Anzola Carlos, Mariuba Luís André Morais, Brito Walter Ricardo
Аннотация
BACKGROUND: The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections was a serious disease that spread rapidly around the world and led to a state of global health emergency. During the pandemic, millions of deaths were notified as result of the progression of the disease to a serious condition. Research into the development of diagnostic tests was very important for the identification and control of new cases.
OBJECTIVES: In this work a label-free electrochemical platform was developed for sensing of SARS-CoV-2 S-protein.
METHODS: The S- antibodies (IgY type) from egg yolk were immobilised though stable bonding onto screen-printed gold electrodes surface, which was previously modified with self-assembled monolayers of cysteamine (Cys). The analytical performance of the devices was followed by differential pulse voltammetry after incubation in various concentrations of S-protein.
FINDINGS: The electrical response exhibited a linear behaviour from 10 to 1000 ng mL-1 [with limit of detection (LOD) of 6.2 ng mL-1]. Also, we confirmed that our method is more sensitive than an enzyme-linked immuno-sorbent assay (ELISA), which was conducted with the same molecules (antibody and antigen) (500-4000 ng mL-1, with LOD = 235 ng mL-1). The immunosensor was selective for S-protein detection, and no significative changes were registered by differential pulse voltammetry in presence of SARS-CoV-2 N-protein. Tests on saliva samples recorded similar results to S protein standards.
MAIN CONCLUSIONS: The developed immunosensor showed good performance and selectivity, therefore, it can be an alternative method for coronavirus disease 2019 (Covid-19) detecting in saliva samples.
OBJECTIVES: In this work a label-free electrochemical platform was developed for sensing of SARS-CoV-2 S-protein.
METHODS: The S- antibodies (IgY type) from egg yolk were immobilised though stable bonding onto screen-printed gold electrodes surface, which was previously modified with self-assembled monolayers of cysteamine (Cys). The analytical performance of the devices was followed by differential pulse voltammetry after incubation in various concentrations of S-protein.
FINDINGS: The electrical response exhibited a linear behaviour from 10 to 1000 ng mL-1 [with limit of detection (LOD) of 6.2 ng mL-1]. Also, we confirmed that our method is more sensitive than an enzyme-linked immuno-sorbent assay (ELISA), which was conducted with the same molecules (antibody and antigen) (500-4000 ng mL-1, with LOD = 235 ng mL-1). The immunosensor was selective for S-protein detection, and no significative changes were registered by differential pulse voltammetry in presence of SARS-CoV-2 N-protein. Tests on saliva samples recorded similar results to S protein standards.
MAIN CONCLUSIONS: The developed immunosensor showed good performance and selectivity, therefore, it can be an alternative method for coronavirus disease 2019 (Covid-19) detecting in saliva samples.