Biomedica : revista del Instituto Nacional de Salud · 2022
Monkeypox virus genome sequence from an imported human case in Colombia.
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Laiton-Donato Katherine, Álvarez-Díaz Diego A, Franco-Muñoz Carlos, Ruiz-Moreno Héctor A, Rojas-Estévez Paola, Prada Andrés, Rosales Alicia, Ospina Martha Lucía, Mercado-Reyes Marcela
Аннотация
INTRODUCTION: Monkeypox virus (MPXV) is an enveloped double-stranded DNA virus with a genome of approximately 197.209 bp. The current classification divides MPXV into three clades: Clade I (Central African or Congo Basin clade) and clades IIa and IIb (West African clades).
OBJECTIVE: To report the complete genome and phylogenetic analysis of a human monkeypox case detected in Colombia.
MATERIALS AND METHODS: Exudate from vesicular lesions was obtained from a male patient with recent travel history to Spain. A direct genomic approach was implemented in which total DNA from the sample was purified through a column-based method, followed by sequencing on the Nanopore GridION. Reads were aligned against the MPXV reference genome using minimap2 v.2.24 and phylogenetic inference was performed using maximum likelihood estimation.
RESULTS: A total of 11.951 reads mapped directly to a reference genome with 96.8% of coverage (190.898 bp).
CONCLUSION: Phylogenetic analysis of the MPXV circulating in Colombia demonstrated its close relationship to clade IIb responsible for the multi-country outbreak in 2022.
OBJECTIVE: To report the complete genome and phylogenetic analysis of a human monkeypox case detected in Colombia.
MATERIALS AND METHODS: Exudate from vesicular lesions was obtained from a male patient with recent travel history to Spain. A direct genomic approach was implemented in which total DNA from the sample was purified through a column-based method, followed by sequencing on the Nanopore GridION. Reads were aligned against the MPXV reference genome using minimap2 v.2.24 and phylogenetic inference was performed using maximum likelihood estimation.
RESULTS: A total of 11.951 reads mapped directly to a reference genome with 96.8% of coverage (190.898 bp).
CONCLUSION: Phylogenetic analysis of the MPXV circulating in Colombia demonstrated its close relationship to clade IIb responsible for the multi-country outbreak in 2022.