Epigenomics · 2018
Reciprocal changes of H3K27ac and H3K27me3 at the promoter regions of the critical genes for endometrial decidualization.
DatasetСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Katoh Noriko, Kuroda Keiji, Tomikawa Junko, Ogata-Kawata Hiroko, Ozaki Rie, Ochiai Asako, Kitade Mari, Takeda Satoru, Nakabayashi Kazuhiko, Hata Kenichiro
Аннотация
AIM: Decidualization is essential for embryo implantation and placental development. We aimed to obtain transcriptome and epigenome profiles for primary endometrial stromal cells (ESCs) and in vitro decidualized cells.
MATERIALS & METHODS: ESCs isolated from human endometrial tissues remained untreated (D0), or decidualized for 4 days (D4) and 8 days (D8) in the presence of 8-bromo-cAMP and progesterone.
RESULTS: Among the epigenetic modifications examined (DNA methylation, H3K27ac, H3K9me3 and H3K27me3), the H3K27ac patterns changed most dramatically, with a moderate correlation with gene expression changes, upon decidualization. Subsets of up- and down-regulated genes upon decidualization were associated with reciprocal changes of H3K27ac and H3K27me3 modifications at their promoter region, and were enriched with genes essential for decidualization such as WNT4, ZBTB16, PROK1 and GREB1.
CONCLUSION: Our dataset is useful to further elucidate the molecular mechanisms underlying decidualization.
MATERIALS & METHODS: ESCs isolated from human endometrial tissues remained untreated (D0), or decidualized for 4 days (D4) and 8 days (D8) in the presence of 8-bromo-cAMP and progesterone.
RESULTS: Among the epigenetic modifications examined (DNA methylation, H3K27ac, H3K9me3 and H3K27me3), the H3K27ac patterns changed most dramatically, with a moderate correlation with gene expression changes, upon decidualization. Subsets of up- and down-regulated genes upon decidualization were associated with reciprocal changes of H3K27ac and H3K27me3 modifications at their promoter region, and were enriched with genes essential for decidualization such as WNT4, ZBTB16, PROK1 and GREB1.
CONCLUSION: Our dataset is useful to further elucidate the molecular mechanisms underlying decidualization.
Предметные рубрики MeSH
Cells, CulturedDNA MethylationDeciduaEmbryo ImplantationEpigenesis, GeneticFemaleGastrointestinal HormonesHistonesHumansNeoplasm ProteinsPlacentationPregnancyPromoter Regions, GeneticPromyelocytic Leukemia Zinc Finger ProteinStromal CellsVascular Endothelial Growth Factor, Endocrine-Gland-DerivedWnt4 Protein