Zhonghua yi xue za zhi · 1998
[Production of purified Japanese encephalitis vaccine from Vero cells with roller bottles].
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Ding Z, Shi H, Pang C
Аннотация
OBJECTIVE: To study the production process of purified Japanese encephalitis (JE) vaccine from Vero cells cultivated in roller bottles to improve the quality of JE vaccine.
METHODS: The 15 L roller bottles were used for propagation of Vero cells and JE virus, then the virus was inactivated, concentrated, treated by protamine sulphate, purified by sucrose gradient density centrifugation and lyophylized as final product.
RESULTS: Three batches of high quality lyophylized vaccine were produced and the quality control tests of vaccine for human use had been passed.
CONCLUSION: Using roller bottles to cultivate continuous cell line-Vero cells for JE vaccine production is feasible.
METHODS: The 15 L roller bottles were used for propagation of Vero cells and JE virus, then the virus was inactivated, concentrated, treated by protamine sulphate, purified by sucrose gradient density centrifugation and lyophylized as final product.
RESULTS: Three batches of high quality lyophylized vaccine were produced and the quality control tests of vaccine for human use had been passed.
CONCLUSION: Using roller bottles to cultivate continuous cell line-Vero cells for JE vaccine production is feasible.