Expression, purification, and structural analysis of intracellular C-termini from metabotropic glutamate receptors.

Glutamate is the most important excitatory neurotransmitter in the mammalian central nervous system (CNS). Metabotropic glutamate receptors (mGluRs) are G-protein-coupled receptors (GPCRs) that guide several intracellular signal cascades thereby controlling multiple physiological tasks, such as neuronal excitability, learning, and memory. Consequently, these receptors are discussed in the context of several CNS-associated diseases, including addiction for drugs, Alzheimer's disease, Fragile X syndrome, night blindness, or schizophrenia. Although increasing structural information is available for the extracellular and transmembrane domains of GPCRs, data describing the nature of intracellular receptor domains are largely missing. Indeed, in all available crystal structures of neurotransmitter receptors, their intracellular domains were omitted. Most intracellular mGluR C-termini are alternatively spliced and contain multiple binding sites for interacting proteins. Therefore, analyzing their structure can identify molecular mechanisms of receptor regulation. Recently, we analyzed the conformation of the intracellular C-termini of mGluR6, mGluR7a, and mGluR8a. Here, we describe an array of biochemical, biophysical, and computational techniques suited to elucidate the nature of these highly interesting receptor domains.