Tropical medicine & international health : TM & IH · 2011
Plasma vitamin C assay in women of reproductive age in Kampala, Uganda, using a colorimetric method.
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Kiondo Paul, Tumwesigye Nazarius M, Wandabwa Julius, Wamuyu-Maina Gakenia, Bimenya Gabriel S, Okong Pius
Аннотация
OBJECTIVE: Vitamin C alone or in combination with vitamin E has been proposed to prevent pre-eclampsia. In this study, we assayed the plasma vitamin C in women of reproductive age in Kampala and assessed its association with pre-eclampsia.
METHODS: Participants in this study were 215 women with pre-eclampsia, 400 women with normal pregnancy attending antenatal clinic and 200 non-pregnant women attending family planning clinic at Mulago Hospital's Department of Obstetrics and Gynaecology from 1st May 2008 to 1st May 2009. Plasma vitamin C was assayed using the acid phosphotungstate method; differences in the means of plasma vitamin C were determined by ANOVA.
RESULTS: Mean plasma vitamin C levels were 1.72 (SD 0.68)×10(3)μg/l in women with pre-eclampsia, 1.89 (SD 0.73)×10(3)μg/l in women with normal pregnancy and 2.64 (SD 0.97)×10(3)μg/l in non-pregnant women. Plasma vitamin C was lower in women with pre-eclampsia than in women with normal pregnancy (P=0.005) and non-pregnant women (P<0.001).
CONCLUSION: Health workers need to advise women of reproductive age on foods that are rich in vitamin C, as this may improve the vitamin status and possibly reduce the occurrence of pre-eclampsia.
METHODS: Participants in this study were 215 women with pre-eclampsia, 400 women with normal pregnancy attending antenatal clinic and 200 non-pregnant women attending family planning clinic at Mulago Hospital's Department of Obstetrics and Gynaecology from 1st May 2008 to 1st May 2009. Plasma vitamin C was assayed using the acid phosphotungstate method; differences in the means of plasma vitamin C were determined by ANOVA.
RESULTS: Mean plasma vitamin C levels were 1.72 (SD 0.68)×10(3)μg/l in women with pre-eclampsia, 1.89 (SD 0.73)×10(3)μg/l in women with normal pregnancy and 2.64 (SD 0.97)×10(3)μg/l in non-pregnant women. Plasma vitamin C was lower in women with pre-eclampsia than in women with normal pregnancy (P=0.005) and non-pregnant women (P<0.001).
CONCLUSION: Health workers need to advise women of reproductive age on foods that are rich in vitamin C, as this may improve the vitamin status and possibly reduce the occurrence of pre-eclampsia.