Addressing personal protective equipment (PPE) decontamination: Methylene blue and light inactivates severe acute respiratory coronavirus virus 2 (SARS-CoV-2) on N95 respirators and medical masks with maintenance of integrity and fit.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
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Lendvay Thomas Sean, Chen James, Harcourt Brian H, Scholte Florine E M, Lin Ying Ling, Kilinc-Balci F Selcen, Lamb Molly M, Homdayjanakul Kamonthip, Cui Yi, Price Amy, Heyne Belinda, Sahni Jaya, Kabra Kareem B, Lin Yi-Chan, Evans David, Mores Christopher N, Page Ken, Chu Larry F, Haubruge Eric, Thiry Etienne, Ludwig-Begall Louisa F, Wielick Constance, Clark Tanner, Wagner Thor, Timm Emily, Gallagher Thomas, Faris Peter, Macia Nicolas, Mackie Cyrus J, Simmons Sarah M, Reader Susan, Malott Rebecca, Hope Karen, Davies Jan M, Tritsch Sarah R, Dams Lorène, Nauwynck Hans, Willaert Jean-Francois, De Jaeger Simon, Liao Lei, Zhao Mervin, Laperre Jan, Jolois Olivier, Smit Sarah J, Patel Alpa N, Mayo Mark, Parker Rod, Molloy-Simard Vanessa, Lemyre Jean-Luc, Chu Steven, Conly John M, Chu May C
Аннотация
DESIGN: The 2 arms of the study included (1) PPE inoculation with coronaviruses followed by MB with light (MBL) decontamination treatment and (2) PPE treatment with MBL for 5 cycles of decontamination to determine maintenance of PPE performance.
METHODS: MBL treatment was used to inactivate coronaviruses on 3 N95 filtering facepiece respirator (FFR) and 2 medical mask models. We inoculated FFR and medical mask materials with 3 coronaviruses, including SARS-CoV-2, and we treated them with 10 µM MB and exposed them to 50,000 lux of white light or 12,500 lux of red light for 30 minutes. In parallel, integrity was assessed after 5 cycles of decontamination using multiple US and international test methods, and the process was compared with the FDA-authorized vaporized hydrogen peroxide plus ozone (VHP+O3) decontamination method.
RESULTS: Overall, MBL robustly and consistently inactivated all 3 coronaviruses with 99.8% to >99.9% virus inactivation across all FFRs and medical masks tested. FFR and medical mask integrity was maintained after 5 cycles of MBL treatment, whereas 1 FFR model failed after 5 cycles of VHP+O3.
CONCLUSIONS: MBL treatment decontaminated respirators and masks by inactivating 3 tested coronaviruses without compromising integrity through 5 cycles of decontamination. MBL decontamination is effective, is low cost, and does not require specialized equipment, making it applicable in low- to high-resource settings.