La Tunisie medicale · 2008
[Methicillin resistant Staphylococcus aureus: phenotypic and genotypic studies].
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Jemili-Ben Jomaa M, Boutiba-Ben Boubaker I, Ben Redjeb Saïda
Аннотация
BACKGROUND: Staphylococcus aureus is a human opportunistic pathogen. Its important pathogenicity and the increasingly rate of resistance to methicillin are the main causes of morbidity and mortality.
AIM: In order to evaluate the epidemiologic situation of Methicillin Resistant S. aureus (MRSA) at Charles Nicolle hospital.
METHODS: A four years retrospective study (January 1999-December 2002) was conducted.
RESULTS: 65 non redundant MRSA isolates were collected. Identification was based on morphology, culture and biochemical characters. Antibiotic susceptibility was determined by disk diffusion method. Resistance to methicillin was confirmed by mec A PCR. Molecular typing was performed by Random Amplified Polymorphic DNA using ERIC-IR. Despite a perfect biotypic similarity between strains, ERIC-IR PCR revealed 7 genotypes.
CONCLUSION: The combination of phenotypic methods and RAPD fingerprinting were easy to perform routinely for MRSA typing. However, phylogenetic relationship between strains needs more investigations.
AIM: In order to evaluate the epidemiologic situation of Methicillin Resistant S. aureus (MRSA) at Charles Nicolle hospital.
METHODS: A four years retrospective study (January 1999-December 2002) was conducted.
RESULTS: 65 non redundant MRSA isolates were collected. Identification was based on morphology, culture and biochemical characters. Antibiotic susceptibility was determined by disk diffusion method. Resistance to methicillin was confirmed by mec A PCR. Molecular typing was performed by Random Amplified Polymorphic DNA using ERIC-IR. Despite a perfect biotypic similarity between strains, ERIC-IR PCR revealed 7 genotypes.
CONCLUSION: The combination of phenotypic methods and RAPD fingerprinting were easy to perform routinely for MRSA typing. However, phylogenetic relationship between strains needs more investigations.