Journal of ethnopharmacology · 2025
The Chinese herbal formular Kang Shuai Lao Pian enhances health status for skin, adipose-derived stem cells, intestinal microbes and metabolites in naturally senescent mice.
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Li Minrui, Yan Ziwei, Hua Wentian, Gong Shuqing, Wang Yi, Chen Huan, Zhang Shujing, Qian Jing
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Aging is a time-dependent decline in physiological function that reduces quality of life. In traditional Chinese medicine (TCM), aging is viewed as "deficiency" complicated by blood stasis and qi stagnation. "Kang Shuai Lao Pian" (KSLP) is a Ming Dynasty herbal formula widely used to repenish qi, nourish yin, calm the heart, and tranquilize the mind.
AIM OF THE STUDY: We aimed to evaluate the systemic anti-aging activity of KSLP and to determine its mode of action in naturally senescent mice.
MATERIALS AND METHODS: Male 16-18-month-old mice received daily intragastric gavage of KSLP (450 mg/kg) for 8 weeks. Skin structure, adipose distribution, neurotransmitters, adipose-derived stem cells (ADSCs) morphology and differentiation, 16S rRNA gene sequencing and untargeted metabolomics analysis of cecal feces were assessed. RNA sequencing (RNA-Seq) was performed on senescent ADSCs; metabolic-microbe correlations were analyzed by Pearson correlation.
RESULTS: KSLP restored skin structure and dermal collagen density, normalized dopamine, norepinephrine, acetylcholine, and 5-hydroxytryptamine levels, and improved ADSCs osteogenic/adipogenic capacity- changes mechanistically linked to PI3K-Akt and cytokine-cytokine receptor interaction signaling pathways. In gut, KSLP reversed age-associated shifts in Firmicutes, Epsilonbacteraeota, Ruminococcaceae, and Helicobacter and modulated 57 metabolites particularly within amino acid, fatty acid, and nucleotide metabolic pathways. Correlation analysis identified Ruminococcaceae_UCG_014, Candidatus_Saccharimonas, Helicobacter, Rikenellaceae_RC9, and Ileibacterium as keystone taxa linked to KSLP-responsive metabolites.
CONCLUSION: KSLP systemically improves skin architecture, ADSCs function, gut-microbiome balance, and metabolic profiles in naturally aged mice, supporting its clinical potential against age-related decline. Bioactivity-guided identification of active compounds and in vivo mechanistic validation are warranted.
AIM OF THE STUDY: We aimed to evaluate the systemic anti-aging activity of KSLP and to determine its mode of action in naturally senescent mice.
MATERIALS AND METHODS: Male 16-18-month-old mice received daily intragastric gavage of KSLP (450 mg/kg) for 8 weeks. Skin structure, adipose distribution, neurotransmitters, adipose-derived stem cells (ADSCs) morphology and differentiation, 16S rRNA gene sequencing and untargeted metabolomics analysis of cecal feces were assessed. RNA sequencing (RNA-Seq) was performed on senescent ADSCs; metabolic-microbe correlations were analyzed by Pearson correlation.
RESULTS: KSLP restored skin structure and dermal collagen density, normalized dopamine, norepinephrine, acetylcholine, and 5-hydroxytryptamine levels, and improved ADSCs osteogenic/adipogenic capacity- changes mechanistically linked to PI3K-Akt and cytokine-cytokine receptor interaction signaling pathways. In gut, KSLP reversed age-associated shifts in Firmicutes, Epsilonbacteraeota, Ruminococcaceae, and Helicobacter and modulated 57 metabolites particularly within amino acid, fatty acid, and nucleotide metabolic pathways. Correlation analysis identified Ruminococcaceae_UCG_014, Candidatus_Saccharimonas, Helicobacter, Rikenellaceae_RC9, and Ileibacterium as keystone taxa linked to KSLP-responsive metabolites.
CONCLUSION: KSLP systemically improves skin architecture, ADSCs function, gut-microbiome balance, and metabolic profiles in naturally aged mice, supporting its clinical potential against age-related decline. Bioactivity-guided identification of active compounds and in vivo mechanistic validation are warranted.