Chromosome-borne bla CTX-M-65 gene in non-O1/O139 Vibrio cholerae isolated from bile.

BACKGROUND AND

OBJECTIVES

Non-O1/O139 Vibrio cholerae (NOVC) has been associated with extraintestinal infections; however, its isolation from bile remains exceedingly rare. This study reports the identification and comprehensive analysis of a NOVC strain isolated from bile, including its antimicrobial resistance profile, virulence gene content, and molecular characteristics.

MATERIALS AND

METHODS

A NOVC isolate was obtained from the bile of a patient with a hepatobiliary tumor. The isolate was identified and subjected to antimicrobial susceptibility testing. Whole-genome sequencing was performed to characterize its molecular features, including antimicrobial resistance genes, virulence genes, and relevant genetic mutations.

RESULTS

The NOVC isolate presents a multi-drug resistance phenotype. Corresponding genomic analysis indicates that this strain belongs to a novel sequence type (ST1736), carrying various drug resistance genes and virulence factors. Moreover, the bla CTX-M-65 extended-spectrum β-lactamase (ESBL) gene was detected in its chromosomal genome.

CONCLUSION

This study presents the first report of a multidrug-resistant NOVC strain isolated from bile in mainland China. Notably, the ESBL gene bla CTX-M-65 was identified chromosomally in NOVC for the first time.