Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego · 2021
Efficacy of evaluation of microRNA-122 and microRNA-21 levels in the role of biomarkers of oligosymptomatic choledocholithiasis in patients with acute calculous cholecystitis.
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Kolomiytsev Vasyl, Terletskiy Oleg, Bufan Mariya, Lukavetskiy Oleksiy
Аннотация
AIM: The aim of this study was to evaluate the efficacy of determining the microRNA-122 and microRNA-21 expression levels in serum and bile as predictors of the presence of oligosymptomatic choledocholithiasis in patients with acute calculous cholecystitis.
MATERIALS AND METHODS: The study included 32 patients who were divided into two groups: I - study group and II - comparison group. The study group included patients with acute calculous cholecystitis (ACC) and oligosymptomatic choledocholithiasis (OCL) (n=23), and the comparison group consisted of patients with ACC without choledocholithiasis (n=9). Conditionally healthy people (volunteers, n=20) who made up the third group.
RESULTS: Comparison analysis of the expression levels of the studied microRNAs in all clinical groups revealed statistically significant differences for both microRNA-122 and microRNA-21. As a result of ROC analysis, the multivariate model for the prediction of OCL presence had better characteristics (AUC=0.95 vs. AUC=0.90) when compared to the univariate test for determining the microRNA-122 level in bile. The cut-off threshold for the presence or absence of OCL (cut-off) is equal to 77. Values higher than the given are regarded as the presence of OCL, and the lower ones indicate the absence of it. The sensitivity of this model was 87.0%, specificity - 100%, accuracy - 90.6%.
CONCLUSIONS: The detected changes in the levels of microRNA-122 and microRNA-21 in serum and bile indicate the possibility of using them as a diagnostic marker of oligosymptomatic choledocholithiasis. The increase in expression of microRNA-122 and microRNA-21 in serum is likely (p<0.05) to be associated with high expression levels in bile. The probability model of the presence of oligosymptomatic choledocholithiasis in patients with acute calculous cholecystitis using quantitative determination of expression levels of microRNA-122 and microRNA-21 in serum and bile has high prognostic significance and can be recommended for application in clinical protocols of patient examination.
MATERIALS AND METHODS: The study included 32 patients who were divided into two groups: I - study group and II - comparison group. The study group included patients with acute calculous cholecystitis (ACC) and oligosymptomatic choledocholithiasis (OCL) (n=23), and the comparison group consisted of patients with ACC without choledocholithiasis (n=9). Conditionally healthy people (volunteers, n=20) who made up the third group.
RESULTS: Comparison analysis of the expression levels of the studied microRNAs in all clinical groups revealed statistically significant differences for both microRNA-122 and microRNA-21. As a result of ROC analysis, the multivariate model for the prediction of OCL presence had better characteristics (AUC=0.95 vs. AUC=0.90) when compared to the univariate test for determining the microRNA-122 level in bile. The cut-off threshold for the presence or absence of OCL (cut-off) is equal to 77. Values higher than the given are regarded as the presence of OCL, and the lower ones indicate the absence of it. The sensitivity of this model was 87.0%, specificity - 100%, accuracy - 90.6%.
CONCLUSIONS: The detected changes in the levels of microRNA-122 and microRNA-21 in serum and bile indicate the possibility of using them as a diagnostic marker of oligosymptomatic choledocholithiasis. The increase in expression of microRNA-122 and microRNA-21 in serum is likely (p<0.05) to be associated with high expression levels in bile. The probability model of the presence of oligosymptomatic choledocholithiasis in patients with acute calculous cholecystitis using quantitative determination of expression levels of microRNA-122 and microRNA-21 in serum and bile has high prognostic significance and can be recommended for application in clinical protocols of patient examination.