Molecules (Basel, Switzerland) · 2012
Biochemical characterization of an in-house Coccidioides antigen: perspectives for the immunodiagnosis of coccidioidomycosis.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Filho Renato Evando Moreira, Bandeira Silviane Praciano, Brillhante Raimunda Sâmia Nogueira, Rocha Marcos Fábio Gadelha, Vasconcelos Ilka Maria, Pereira Mirella Leite, Castelo-Branco Débora de Souza Collares Maia, Rocha Francisco Airton Castro, Camargo Zoilo Pires de, Ramos Marcio Viana, Cordeiro Rossana de Aguiar, Sidrim José Júlio Costa
Аннотация
The objective of this study was to evaluate the reactivity of an in-house antigen, extracted from a strain of C. posadasii isolated in northeastern Brazil, by radial immunodiffusion and Western blotting, as well as to establish its biochemical characterization. The protein antigen was initially extracted with the use of solid ammonium sulfate and characterized by 1-D electrophoresis. Subsequently, it was tested by means of double radial immunodiffusion and Western blotting. A positive reaction was observed against the antigen by both immunodiagnostic techniques tested on sera from patients suffering from coccidioidomycosis. Besides this, two immunoreactive protein bands were observed and were revealed to be a β-glucosidase and a glutamine synthetase after sequencing of the respective N-terminal regions. Our in-house Coccidioides antigen can be promising as a quick and low-cost diagnostic tool without the risk of direct manipulation of the microorganism.