[Search for Fanconi anemia/BRCA pathway defects in lymphoma cell lines].

OBJECTIVE

To investigate the possible relationship between defects in the FA/BRCA pathway of genomic stability and potential pathogenesis of T and B cell lymphoma.

METHODS

Nineteen cell lines derived from diverse subtypes of lymphoma for possible FA pathway defects were screened.

RESULTS

No defect in FANCD2 ubiquitination was observed. However, the FANCN protein was absent in cell lines HT and Sudhl4. This absence was correlated with enhanced MMC-induced G2 arrest, growth inhibition and high chromosomal breakage rate in both cell lines. In addition, in exon-5a of FANCN gene, a mutation of c.1769 C>T, p. A590V was found in cell line HT, but not in cell line Sudhl4.

CONCLUSION

This mutation may be the reason causing the absence of the FANCN protein expression or making the protein unstable and losing its function.