Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2005
[Mechanism of apoptin-induced apoptosis of human lymphoma cells U937].
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Ben Song-bin, Zhao Hong-li, Nie Jing, Li Hong-yang
Аннотация
AIM: To study the role of c-Jun N-terminal kinase (JNK) in the apoptosis induced by apoptin gene in human lymphoma cell U937.
METHODS: The U937 cells were transiently transfected by pcDNAA3 plasmids containing apoptin gene. Apoptosis of U937 cells was measured by flow-cytometry. Activation of JNK signal pathway was detected by Western blot.
RESULTS: Apoptin could induce apoptosis of U937 cells in vitro at 48 h after transfection. The level of phosphorylated JNK was increased at 24 h and reached the peak level at 48 h after transfection.
CONCLUSION: Apoptin gene can induce apoptosis of U937 cells in which JNK signal pathway plays an important role.
METHODS: The U937 cells were transiently transfected by pcDNAA3 plasmids containing apoptin gene. Apoptosis of U937 cells was measured by flow-cytometry. Activation of JNK signal pathway was detected by Western blot.
RESULTS: Apoptin could induce apoptosis of U937 cells in vitro at 48 h after transfection. The level of phosphorylated JNK was increased at 24 h and reached the peak level at 48 h after transfection.
CONCLUSION: Apoptin gene can induce apoptosis of U937 cells in which JNK signal pathway plays an important role.