Revista da Sociedade Brasileira de Medicina Tropical · 2010
A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples.
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Koishi Andrea Cristine, Vituri Débora Fonseca, Dionízio Filho Pedro Sebastião Raimundo, Sasaki Alexandre Augusto, Felipe Maria Sueli Soares, Venancio Emerson José
Аннотация
INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis.
METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction. The semi-nested PCR was used to investigate biopsies of five patients with oral lesions that resembled paracoccidioidomycosis.
RESULTS: The semi-nested PCR was positive for four samples and negative for a sample from a patient later diagnosed with leishmaniasis.
CONCLUSIONS: The new semi-nested PCR describe is useful for paracoccidioidomycosis diagnosis.
METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction. The semi-nested PCR was used to investigate biopsies of five patients with oral lesions that resembled paracoccidioidomycosis.
RESULTS: The semi-nested PCR was positive for four samples and negative for a sample from a patient later diagnosed with leishmaniasis.
CONCLUSIONS: The new semi-nested PCR describe is useful for paracoccidioidomycosis diagnosis.