Fertility and sterility · 2012
Degradation of actin nucleators affects cortical polarity of aged mouse oocytes.
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Sun Shao-Chen, Gao Wei-Wei, Xu Yong-Nan, Jin Yong-Xun, Wang Qing-Ling, Yin Xi-Jun, Cui Xiang-Shun, Kim Nam-Hyung
Аннотация
OBJECTIVE: To investigate the molecular mechanism of mouse oocyte polarity loss during aging.
DESIGN: Experimental study.
SETTING: Academic basic research laboratory.
ANIMAL(S): Mice.
INTERVENTION(S): Oocytes were collected 16 hours after injection of hCG and cultured in M16 medium for an additional 14 hours with or without caffeine.
MAIN OUTCOME MEASURE(S): Expression and localizations of actin nucleators actin-related protein 2/3 complex, JMY, and WAVE2 were examined by immunofluorescence staining, and their messenger RNA levels were examined by real-time reverse transcription-polymerase chain reaction.
RESULT(S): The protein and messenger RNA levels of actin-related protein 2/3 complex, JMY, and WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes.
CONCLUSION(S): Our data indicated that the loss of oocyte polarity may be due to the degradation of actin nucleators in aged oocytes.
DESIGN: Experimental study.
SETTING: Academic basic research laboratory.
ANIMAL(S): Mice.
INTERVENTION(S): Oocytes were collected 16 hours after injection of hCG and cultured in M16 medium for an additional 14 hours with or without caffeine.
MAIN OUTCOME MEASURE(S): Expression and localizations of actin nucleators actin-related protein 2/3 complex, JMY, and WAVE2 were examined by immunofluorescence staining, and their messenger RNA levels were examined by real-time reverse transcription-polymerase chain reaction.
RESULT(S): The protein and messenger RNA levels of actin-related protein 2/3 complex, JMY, and WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes.
CONCLUSION(S): Our data indicated that the loss of oocyte polarity may be due to the degradation of actin nucleators in aged oocytes.
Предметные рубрики MeSH
Actin-Related Protein 2-3 ComplexActinsAnimalsCaffeineCell Cycle ProteinsCell PolarityCellular SenescenceChorionic GonadotropinFemaleFertility Agents, FemaleFluorescent Antibody TechniqueGene Expression RegulationMiceMice, Inbred ICRNuclear ProteinsOocytesOvulation InductionRNA, MessengerReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionTime FactorsTrans-ActivatorsWiskott-Aldrich Syndrome Protein Family