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DOI 10.1016/j.fertnstert.2012.01.101

Медицинская библиотека · Fertility and sterility · 2012

Degradation of actin nucleators affects cortical polarity of aged mouse oocytes.

Sun Shao-Chen, Gao Wei-Wei, Xu Yong-Nan, Jin Yong-Xun, Wang Qing-Ling, Yin Xi-Jun, Cui Xiang-Shun, Kim Nam-Hyung

СтатьяПоддержка исследования: вне правительства СШАПеревод пока не подготовлен

Аннотация

OBJECTIVE

To investigate the molecular mechanism of mouse oocyte polarity loss during aging.

DESIGN

Experimental study.

SETTING

Academic basic research laboratory.

ANIMAL(S): Mice.

INTERVENTION(S): Oocytes were collected 16 hours after injection of hCG and cultured in M16 medium for an additional 14 hours with or without caffeine.

MAIN OUTCOME MEASURE(S): Expression and localizations of actin nucleators actin-related protein 2/3 complex, JMY, and WAVE2 were examined by immunofluorescence staining, and their messenger RNA levels were examined by real-time reverse transcription-polymerase chain reaction.

RESULT(S): The protein and messenger RNA levels of actin-related protein 2/3 complex, JMY, and WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes.

CONCLUSION(S): Our data indicated that the loss of oocyte polarity may be due to the degradation of actin nucleators in aged oocytes.

Для цитирования: Sun Shao-Chen, Gao Wei-Wei, Xu Yong-Nan, Jin Yong-Xun, Wang Qing-Ling, Yin Xi-Jun, Cui Xiang-Shun, Kim Nam-Hyung. Degradation of actin nucleators affects cortical polarity of aged mouse oocytes.. Fertility and sterility. 2012. DOI: 10.1016/j.fertnstert.2012.01.101.

Предметные рубрики MeSH

Actin-Related Protein 2-3 ComplexActinsAnimalsCaffeineCell Cycle ProteinsCell PolarityCellular SenescenceChorionic GonadotropinFemaleFertility Agents, FemaleFluorescent Antibody TechniqueGene Expression RegulationMiceMice, Inbred ICRNuclear ProteinsOocytesOvulation InductionRNA, MessengerReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionTime FactorsTrans-ActivatorsWiskott-Aldrich Syndrome Protein Family
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