Experimental model of small-for-size syndrome in pigs.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
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Alvarez Paola Sofia Espinoza, DE Lá Hoz Jhosimar Alvarez, Waisberg Daniel Reis, Belon Alessandro Rodrigo, Lima Larissa Barbosa, Soares Beatriz de Lima, Gonzalez Ricardo Luis Santamaria, Arantes Rubens Macedo, Santos João Paulo Costa Dos, Cassino Pedro Carvalho, Ferreira Cinthia Lanchotte, Kubrusly Marcia Saldanha, Brandão Luciana Severo, Rocha-Santos Vinicius, Ducatti Liliana, Pinheiro Rafael Soares, Castro Pedro Marin, Martino Rodrigo Bronze de, Haddad Luciana Bertocco, Nacif Lucas Souto, Galvão Flavio Henrique Ferreira, D'Albuquerque Luiz Augusto Carneiro, Andraus Wellington
Аннотация
AIMS: This study aims to establish a translational model of porcine SFSS, integrating hemodynamic, histopathological, and molecular analyses to quantify the tissue expression of biomarkers to assess liver regeneration.
METHODS: Ten liver transplants were performed: eight with small (partial) for size (donors underwent a 70% hepatectomy) and two with whole liver. The recipients were followed until death (euthanasia due to severe morbidity) or for five days. Hemodynamic data, laboratory analyses, and liver tissue biopsies were collected. Immunohistochemistry was performed to assess the expression of TNF-α, IL-1β, NF-κB, and VEGF. Gene expression levels of VEGF, IL-6, IL-10, and NF-κB were quantified using reverse transcription polymerase chain reaction.
RESULTS: In the partial group, survival rates were 75% in the immediate postoperative period, 25% at 48 hours, and 12.5% at five days. Immunohistochemistry showed a significant increase in TNF-α and NF-κB proteins after reperfusion and at the end of surgery, respectively. Gene expression analysis showed a significant increase in IL-6 and IL-10 at the end of surgery.
CONCLUSIONS: This study represents a methodological advance by performing the first integrated evaluation of protein and gene expression (TNF-α, IL-6, IL-1, IL-10, VEGF, and NF-κB) correlated with hemodynamic, histopathological, and functional parameters in the small-for-size pig model.