Topical application of mitomycin C before versus after myringotomy: an experimental study.

The objective of this animal study was to compare topical mitomycin C (MMC) before and after nonlaser myringotomy. Bilateral myringotomies were performed in 12 rats. On the left side, MMC was applied to the intact tympanic membrane for 10 min before myringotomy (preapplication group). On the right side, MMC was applied to the tympanic membrane following myringotomy. Tympanic membranes were observed for a total of 8 weeks. By day 35, all myringotomies with application of MMC after perforation of the tympanic membrane were closed, whereas 42% of the myringotomies with application of MMC prior to perforation remained patent. One tympanic membrane in the preapplication group showed an enlargement of the perforation with partial destruction of the malleus. The present study showed that the application of MMC to the intact tympanic membrane prolongs the patency of nonlaser myringotomies in rats. Specific side effects of the MMC application must be kept in mind.