Galectin-10 in serum extracellular vesicles reflects asthma pathophysiology.
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Yoshimura Hanako, Takeda Yoshito, Shirai Yuya, Yamamoto Makoto, Nakatsubo Daisuke, Amiya Saori, Enomoto Takatoshi, Hara Reina, Adachi Yuichi, Edahiro Ryuya, Yaga Moto, Masuhiro Kentaro, Koba Taro, Itoh-Takahashi Miho, Nakayama Mana, Takata So, Hosono Yuki, Obata Sho, Nishide Masayuki, Hata Akinori, Yanagawa Masahiro, Namba Satoko, Iwata Michio, Hamano Momoko, Hirata Haruhiko, Koyama Shohei, Iwahori Kota, Nagatomo Izumi, Suga Yasuhiko, Miyake Kotaro, Shiroyama Takayuki, Fukushima Kiyoharu, Futami Shinji, Naito Yujiro, Kawasaki Takahiro, Mizuguchi Kenji, Kawashima Yusuke, Yamanishi Yoshihiro, Adachi Jun, Nogami-Itoh Mari, Ueki Shigeharu, Kumanogoh Atsushi
Аннотация
OBJECTIVE: We sought to identify novel BMs reflecting tissue pathology from serum extracellular vesicles (EVs).
METHODS: We performed data-independent acquisition of serum EVs from 4 healthy controls, 4 noneosinophilic asthma (NEA) patients, and 4 eosinophilic asthma (EA) patients to identify novel BMs for BA. We confirmed EA-specific BMs via data-independent acquisition validation in 61 BA patients and 23 controls. To further validate these findings, we performed data-independent acquisition for 6 patients with chronic rhinosinusitis without nasal polyps and 7 patients with chronic rhinosinusitis with nasal polyps.
RESULTS: We identified 3032 proteins, 23 of which exhibited differential expression in EA. Ingenuity pathway analysis revealed that protein signatures from each phenotype reflected disease characteristics. Validation revealed 5 EA-specific BMs, including galectin-10 (Gal10), eosinophil peroxidase, major basic protein, eosinophil-derived neurotoxin, and arachidonate 15-lipoxygenase. The potential of Gal10 in EVs was superior to that of eosinophils in terms of diagnostic capability and detection of airway obstruction. In rhinosinusitis patients, 1752 and 8413 proteins were identified from EVs and tissues, respectively. Among 11 BMs identified in EVs and tissues from patients with chronic rhinosinusitis with nasal polyps, 5 (including Gal10 and eosinophil peroxidase) showed significant correlations between EVs and tissues. Gal10 release from EVs was implicated in eosinophil extracellular trapped cell death in vitro and in vivo.
CONCLUSION: Novel BMs such as Gal10 from serum EVs reflect disease pathophysiology in BA and may represent a new target for liquid biopsy approaches.