Human iPSC-Derived Astrocyte-Endothelial Cell and Neuron-Endothelial Cell Co-culture Models of the Blood-Brain Barrier to Study the Impact of Ischemic Stroke in Vitro.

The role of the blood-brain barrier (BBB) in pathogenic mechanisms of neurodegenerative disorders, including stroke, is well recognized. In vitro BBB models offer opportunities to recapitulate aspects of the disease using different cell types, extracellular matrices, and soluble factors to study the biology of the disease and the role of the BBB. This chapter describes the rigorous protocols and our schemes to successfully establish human-induced pluripotent stem cell-derived astrocyte-endothelial cell and neuron-endothelial cell co-culture models to study the impact of ischemia-reoxygenation for in vitro stroke studies.