HAEC in the treatment of brain hemorrhage: a preliminary observation in rabbits.

OBJECTIVE

This study aimed to evaluate the therapeutic potential of human amniotic epithelial cell (HAEC) transplantation in the management of brain hemorrhage in an animal model.

METHODS

New Zealand white rabbits were induced to develop cerebral hemorrhage through autologous blood injection. Animals with confirmed brain hemorrhage were randomized to receive transplantation of, respectively, vehicle (n=15) and primary HAECs (n=15) that were expressing embryonic stem cell- and neuron-specific markers and were transfected with a retroviral vector carrying the green fluorescent protein (GFP). Behavioral and histological changes, survival of transplanted HAECs, and expression of glial fibrillary acidic protein (GFAP) and MAP-2 in transplanted perifocal tissue were assessed at various time points after transplantation.

RESULTS

At 2-3 weeks after transplantation, walking, body weight-supporting and movement coordinating capacities of limbs were improved mostly level II-III hemorrhage lesion cases in HAEC transplantation group but mostly in level I-II hemorrhage lesion cases in the vehicle control group. The Tarlov scores were significantly difference between the two groups (P<0.05). GFAP- and MAP-2-positive cells were observed in the neural tissue in animals transplanted with hAECs but not in animals in the control group (P<0.05).

CONCLUSION

These preliminary observations suggest that hAEC transplantation possess both embryonic stem cell features and a neuron differentiation potential and thus may offer a promising treatment for hemorrhage-associated neurological damage.